ID   DNAK_FLAJ1              Reviewed;         627 AA.
AC   A5FGL1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=Fjoh_2631;
OS   Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC
OS   14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae).
OC   Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Flavobacterium.
OX   NCBI_TaxID=376686;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101;
RX   PubMed=19717629; DOI=10.1128/aem.01495-09;
RA   McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA   Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA   Cheng Y.Q., Stein J.L.;
RT   "Novel features of the polysaccharide-digesting gliding bacterium
RT   Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL   Appl. Environ. Microbiol. 75:6864-6875(2009).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000685; ABQ05658.1; -; Genomic_DNA.
DR   RefSeq; WP_012024697.1; NZ_MUGZ01000009.1.
DR   AlphaFoldDB; A5FGL1; -.
DR   SMR; A5FGL1; -.
DR   STRING; 376686.Fjoh_2631; -.
DR   EnsemblBacteria; ABQ05658; ABQ05658; Fjoh_2631.
DR   KEGG; fjo:Fjoh_2631; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_10; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   Proteomes; UP000006694; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..627
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000079226"
FT   REGION          596..627
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        598..616
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         197
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   627 AA;  67395 MW;  7CEB178DE4D253E0 CRC64;
     MGKIIGIDLG TTNSCVSVME GNEAVVIPNA EGKRTTPSII AFVEGGEIKV GDPAKRQAVT
     NPTKTIASIK RFMGHTFAET QDEAKRVPYS VVKGDNNTPR VDIDGRLYTA QELSAMTLQK
     MKKTAEDYLG QTVTEAVITV PAYFNDAQRQ ATKEAGEIAG LKVMRIINEP TAAALAYGLD
     KKGNDQKIAV YDLGGGTFDI SVLELGDGVF EVLSTNGDTH LGGDDFDQVI IDWLADEFKT
     EEGIDLRLDP MSLQRLKEAA EKAKIELSSS AETEINLPYV TATASGPKHL VKKLSRAKFE
     QLSDTLVKRS MEPVAKALKD AGLSTSDIDE VILVGGSTRM PRIADEVEKF FGKKASKGVN
     PDEVVAIGAA IQGGVLSGDV KDVLLLDVTP LSLGIETMGG VLTKLIESNT TIPTKKSQVF
     STAADSQPSV EIHVLQGERA MAADNKTIGR FHLDGIPPAP RGVPQIEVTF DIDANGIIKV
     SATDKGTGKS HDIRIEASSG LTAEEIEKMK KDAEANADAD RIAKERAEKL NEADSTIFQT
     ESQLKELGDK LTDDQKTAIE YALTELRMAH QSQDLDAIQK GLDNVNAAWK TATEAMYAQG
     GDQGQQAAPQ QEQSGDNVED VEFEEVK