ID   DNAK_GUITH              Reviewed;         627 AA.
AC   P29215; O78509;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Chaperone protein dnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; Synonyms=ctp70;
OS   Guillardia theta (Cryptophyte) (Cryptomonas phi).
OG   Plastid; Chloroplast.
OC   Eukaryota; Cryptophyceae; Pyrenomonadales; Geminigeraceae; Guillardia.
OX   NCBI_TaxID=55529;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1961745; DOI=10.1073/pnas.88.23.10783;
RA   Wang S., Liu X.-Q.;
RT   "The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a
RT   histone-like protein, and an acyl carrier protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:10783-10787(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9929392; DOI=10.1007/pl00006462;
RA   Douglas S.E., Penny S.L.;
RT   "The plastid genome of the cryptophyte alga, Guillardia theta: complete
RT   sequence and conserved synteny groups confirm its common ancestry with red
RT   algae.";
RL   J. Mol. Evol. 48:236-244(1999).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; AF041468; AAC35702.1; -; Genomic_DNA.
DR   PIR; A41609; A41609.
DR   RefSeq; NP_050768.1; NC_000926.1.
DR   AlphaFoldDB; P29215; -.
DR   SMR; P29215; -.
DR   GeneID; 857076; -.
DR   HOGENOM; CLU_005965_2_4_1; -.
DR   OMA; KRQGVVN; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Chloroplast; Nucleotide-binding; Plastid.
FT   CHAIN           1..627
FT                   /note="Chaperone protein dnaK"
FT                   /id="PRO_0000078608"
FT   REGION          597..627
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        600..621
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        551
FT                   /note="K -> L (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        586
FT                   /note="L -> V (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        604
FT                   /note="E -> Q (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   627 AA;  68466 MW;  18121DDCBFF7033C CRC64;
     MGKVVGIDLG TTNSVVAVME GGKPAVIQNA EGFRTTPSVV AYTKTGDRLV GQIAKRQAVI
     NPDNTFYSVK RFIGRRSEEV SEELKQVSYI VKTDSNGNIK LDCPSLKKEF ASEEISAEVL
     RKLVDDASKY LGESVKQAVI TVPAYFNDSQ RQATKDAGRI AGLEVLRIIN EPTAASLAYG
     LDKKNNETIL VFDLGGGTFD VSVLEVGDGV FEVLSTSGDT HLGGDDFDDK IVQWLLKEFE
     TEHSINLKSD RQALQRLTEA SEKAKIELSN LSQTEINLPF LTATETGPKH LERSITRAKF
     EELCSDLINR VKIPVENALK DAKLDSSKID EVVLVGGSTR IPAIQELVKR ILNKTPNQTV
     NPDEVVAIGA AVQAGVLAGE VKDILLLDVT PLSLGVETLG GVTTRIIPRN TTIPTKKSEV
     FSTAVDNQPN VEIHVLQGER EFAKDNKSLG TFRLDGILPA PRGVPQIEVT FDIDANGILS
     VTAKDKGTGK EQSITITGAS TLPSDEVERM VNEAQNSAKE DKEKRDKIDL KNQSDSLCYQ
     SEKQLKELEG KIDDTNKNKI SSMISELRNA INNENYDEMR DLNSKLQTAL MDLGKSVYEK
     TSKEQTSTSS PTNSNDSVID ADFSETK