ADDB_LACPL
ID ADDB_LACPL Reviewed; 1200 AA.
AC Q88U40; F9URH7;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01453};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01453};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01453};
DE AltName: Full=ATP-dependent helicase/nuclease RexB {ECO:0000255|HAMAP-Rule:MF_01453};
GN Name=rexB {ECO:0000255|HAMAP-Rule:MF_01453}; OrderedLocusNames=lp_2694;
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. This subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01453}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01453};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01453};
CC -!- SUBUNIT: Heterodimer of AddA and RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01453}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01453}.
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DR EMBL; AL935263; CCC79816.1; -; Genomic_DNA.
DR RefSeq; WP_011101883.1; NC_004567.2.
DR RefSeq; YP_004890330.1; NC_004567.2.
DR AlphaFoldDB; Q88U40; -.
DR SMR; Q88U40; -.
DR STRING; 220668.lp_2694; -.
DR PRIDE; Q88U40; -.
DR EnsemblBacteria; CCC79816; CCC79816; lp_2694.
DR KEGG; lpl:lp_2694; -.
DR PATRIC; fig|220668.9.peg.2255; -.
DR eggNOG; COG3857; Bacteria.
DR HOGENOM; CLU_007838_0_0_9; -.
DR PhylomeDB; Q88U40; -.
DR BioCyc; LPLA220668:G1GW0-2307-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR HAMAP; MF_01453; AddB_type2; 1.
DR InterPro; IPR014141; DNA_helicase_suRexB.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Nuclease;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..1200
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379375"
SQ SEQUENCE 1200 AA; 136246 MW; 66DEE2EE16E64461 CRC64;
MSLQFVLGPA KMDHRRTMVA QLVATLMAKP QDQFFYLVPN HIKFDTEVDV LNRLAQAFGQ
PDLYAQTQVQ VFSFTRLAWY LMKNEAAYQV PRLSAAGIDM LLYRILQRHA DELRLFGGEI
SQTGFVTQLA REINELQTAN LQPEDVVQLA ANAQAGDLQA KLHDLAIVYK DFVAATADKY
LKPADILVQL NAYLRRQDLH GTHVYVELAG FAQLPAQEQG IVTTMLEQGA DVTISLMLDH
KVVDRAPENG TLFYQSGRLY YRLYQYARIR QIPVSQDIYA ETPRVAPGLV ALDDFWRGQP
QAATGYEEPN TNVHLFRTDS RQTELAQVGR MIRAMVAKKH ADPADDYHYR DFLIMTRHLD
AYQTMLAPTF HELEIPYFVD LQRSMADHPL VELINALFDI DAQQYQYRDV MRVLKTELLM
PNINGQPMDR QAYRQAVDLT ENFILKSGYH GQRWVQREDW QYFQLTEGDA GVETDQNAEI
SRQINLIHHF VAETLPPFFK KMRQAPDGKA AVTLLVNFLT SQGVTDQLLA WRDQALDRQD
VRAAAEPEQT WQTFCGMLDE YVTILGAEPF EITDFLALLQ AGFEGASYSQ IPSTLDQVLI
SESGMVQSQD HKVVFMVGAT DLVMPDRIMT NNLLSDVDKE NLQLTLSSLD GDHYLNDSAV
VQLGDESCLN YLAFLSARRH LFFSAPLKDD QETDLNWSNY VRRIQRQFNL REHTYLGTPD
PTNADARPFV GTKRRTISHV IQVYRDVLTT NTDRNRVGAP LQPAPVWVWL RQQLTRDPQF
GELARQLMAG LSYRNQPVKL TPASVEALYG HQIYTSISKL EEFYRNQYAY FLKYGLKLRE
RDVFELSAAS TGEFFHAVLD GLIKALRSDQ IPLAQASDQQ LGQYLETVTR QILDQPQFTI
LTSSNRMAYL QRQLINTVRQ IAFAIRNQSK LSAAEPKQTE ILFGNVGKEH GLKALDFQID
ATHSVHVRGK IDRLDQIQVA DQSYLGIVDY KSSQHKFDFQ EAYYGLAMQM LTYLDAVLHN
EQALVGSDQA AVKLAGALYM HIQNPTLKPK DIQGGFEAAL LKKNKYKGIL LDDPQLLEHL
DSELQQGNGT SKVYPFARKK DGSYSGGRAA SLVTNDQLER LLQHNSQLII AAAEAIFAGE
IQLNPIRLND KTTALQYSPY LSIMQFDAML AENAYRDLPP LSAKQVLDLL KTQKGGENHE
