ID   DNAK_HALMT              Reviewed;         625 AA.
AC   Q9HHB9; I3R545;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=HFX_1649;
OS   Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS   14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=523841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA   Kazi A.S., Nair C.K.K.;
RT   "Organization of the DNAK locus of the archeabacterial halophile, Haloferax
RT   mediterranei.";
RL   Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=22843593; DOI=10.1128/jb.00880-12;
RA   Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA   Zhou J., Hu S., Xiang H.;
RT   "Complete genome sequence of the metabolically versatile halophilic
RT   archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT   hydroxyvalerate) producer.";
RL   J. Bacteriol. 194:4463-4464(2012).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; AF069527; AAG23115.1; -; Genomic_DNA.
DR   EMBL; CP001868; AFK19355.1; -; Genomic_DNA.
DR   RefSeq; WP_004056756.1; NZ_CP039139.1.
DR   AlphaFoldDB; Q9HHB9; -.
DR   SMR; Q9HHB9; -.
DR   STRING; 523841.HFX_1649; -.
DR   PRIDE; Q9HHB9; -.
DR   EnsemblBacteria; AFK19355; AFK19355; HFX_1649.
DR   GeneID; 40157007; -.
DR   KEGG; hme:HFX_1649; -.
DR   eggNOG; arCOG03060; Archaea.
DR   HOGENOM; CLU_005965_2_4_2; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 10764at2157; -.
DR   Proteomes; UP000006469; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 2.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding.
FT   CHAIN           1..625
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078595"
FT   REGION          463..532
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          576..625
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        481..525
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        606..625
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   625 AA;  67374 MW;  C3CF4BE0E3573B51 CRC64;
     MASNKILGID LGTTNSAFAV MEGGDPEIIV NAEGDRTTPS VVAFTDDGER LVGKPAKNQA
     IQNPEHTIRS IKRHMGDDGY TVDIEGEEYT PEQISAMILQ KIKRDAEDYL GDEVEKAVIT
     VPAYFNDKQR QATKDAGEIA GLEVERIVNE PTAASMAYGL DDESNQTVLV YDLGGGTFDV
     SVLDLGGGVY EVVATNGDNE LGGDDWDEAI IDWLATEFKN DHGIDLREDR QALQRLKDAA
     EEAKIELSSR KETTINLPFI TATDSGPVHL EYDLTRAKFE SLTKDLIDRT VEPTEQALED
     AGYGKDDIDD VILVGGSTRM PQVLDKVEEI LGSEPKKSVN PDEAVALGAA IQGGVLGGEV
     DDIVLLDVTP LSLGIEVKGG LFERLIEKNT TIPTEESKIF TTAADNQTTV QVRVFQGERE
     MAEDNELLGE FTLSGIPPAP AGTPQIEVGF NIDENGIVNV SAEDKGSGNA ESITIEGGAG
     LSDDEIDRMQ REAEQHAEED KERRRAVEAR NEAEGAVQRA ETLLEENEEN VDDELRADIE
     AAIEDVEAVL EDDDASTDEL EDVTEDLSKE LQEIGKRMYQ QQAQAQAGGP GGAGGPGGAD
     GPGADADPED FVDADADFDD EDDDE