ID   DNAK_LACAC              Reviewed;         614 AA.
AC   Q84BU4; Q5FJP4;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=LBA1247;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SBT2062;
RA   Aoyama K., Uenishi H., Nakajima H.;
RT   "Characterization of Lactobacillus acidophilus dnaK.";
RL   Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; AB059359; BAC66860.1; -; Genomic_DNA.
DR   EMBL; CP000033; AAV43080.1; -; Genomic_DNA.
DR   RefSeq; WP_003547791.1; NC_006814.3.
DR   RefSeq; YP_194111.1; NC_006814.3.
DR   AlphaFoldDB; Q84BU4; -.
DR   SMR; Q84BU4; -.
DR   STRING; 272621.LBA1247; -.
DR   PRIDE; Q84BU4; -.
DR   EnsemblBacteria; AAV43080; AAV43080; LBA1247.
DR   GeneID; 56942838; -.
DR   KEGG; lac:LBA1247; -.
DR   PATRIC; fig|272621.13.peg.1182; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_9; -.
DR   OMA; ISIKRHM; -.
DR   BioCyc; LACI272621:G1G49-1230-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..614
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078472"
FT   REGION          466..505
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          541..562
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          577..614
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        478..505
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         174
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   614 AA;  66323 MW;  EB906E879BB07447 CRC64;
     MSKVIGIDLG TTNSAVAVLE GKEPKIITNP EGNRTTPSVV AFKDGEIQVG EVAKRQAITN
     PNTIVSIKRH MGEADYKVKV GDKSYTPQEI SAFILQYIKK FSEDYLGEEV KDAVITVPAY
     FNDAQRQATK DAGKIAGLNV QRIINEPTAS ALAYGLDKDD DDEKVLVYDL GGGTFDVSVL
     QLGDGVFQVL STNGDTHLGG DDFDNRIMDW LIKNFKDENG VDLSKDKMAM QRLKDASEKA
     KKDLSGVSST HISLPFISAG ESGPLHLEAD LTRAKFDELT NDLVEKTKIP FDNALKDAGL
     TVNDIDKVIL NGGSTRIPAV QKAVKEWAGK EPDHSINPDE AVALGAAIQG GVISGDVKDI
     VLLDVTPLSL GIETMGGVFT KLIDRNTTIP TSKSQIFSTA ADNQPAVDVH VLQGERPMAA
     DDKTLGRFEL TDIPPAPRGV PQIQVTFDID KNGIVNVSAK DMGTGKEQKI TIKSSSGLSD
     EEIKRMQKDA EEHAEEDKKR KDEADLRNEV DQLIFTTEKT LKETKGKVSD EDTKKVQEAL
     DDLKKAQKDN NLDEMKEKKD ALSKAAQDLA VKLYQQNGGA QGAAGQAGPQ GGNPNDGNNG
     GAQDGEFHKV DPNK