ID   DNAK_LACH4              Reviewed;         608 AA.
AC   A8YVQ3;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=lhv_1336;
OS   Lactobacillus helveticus (strain DPC 4571).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=405566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DPC 4571;
RX   PubMed=17993529; DOI=10.1128/jb.01295-07;
RA   Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA   McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA   Beresford T., Ross R.P.;
RT   "Genome sequence of Lactobacillus helveticus: an organism distinguished by
RT   selective gene loss and IS element expansion.";
RL   J. Bacteriol. 190:727-735(2008).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000517; ABX27340.1; -; Genomic_DNA.
DR   RefSeq; WP_012211996.1; NC_010080.1.
DR   AlphaFoldDB; A8YVQ3; -.
DR   SMR; A8YVQ3; -.
DR   STRING; 405566.lhv_1336; -.
DR   EnsemblBacteria; ABX27340; ABX27340; lhv_1336.
DR   KEGG; lhe:lhv_1336; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_9; -.
DR   OMA; ISIKRHM; -.
DR   Proteomes; UP000000790; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..608
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000072036"
FT   REGION          577..608
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        578..592
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         173
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   608 AA;  65701 MW;  3E0DC94D4E3CE4F2 CRC64;
     MSKVIGIDLG TTNSAVAVLE GKEPKIITNP EGNRTTPSVV AFKDGEIQIG EVAKRQAITN
     PNTIVSIKRH MGEADYKVKV GDKEYTPQEI SAFILQYIKK FSEDYLGEKV TDAVITVPAY
     FNDAQRQATK DAGKIAGLNV QRIINEPTAS ALAFGLNKDQ DEKVLVYDLG GGTFDVSVLQ
     LGDGVFQVLS TNGDTHLGGD DFDNRIMDWL IKNFKDENGV DLSKDKMAMQ RLKDAAEKAK
     KDLSGVSSTH ISLPFISAGE AGPLHLEADL TRAKFDELTS DLVEKTKVPF DNALKDAGLT
     VNDIDKVILN GGSTRIPAVQ KAVKEWAGKE PDHSINPDEA VALGAAIQGG VISGDVKDIV
     LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQIFSTAA DNQPAVDIHV LQGERPMAAD
     DKTLGRFELT DIPPAPRGVP QIQVTFDIDK NGIVNVSAKD MGTGKEQKIT IKSSSGLSDE
     EIKRMQKDAE EHAEEDKKRK EEVDLRNEVD QLIFTTDKTL KDTKDKLSDS DRKPVEDALE
     ALKKAQKDNN LDEMKEKKDA LSKAAQDLAV KLYQQNGGAQ GAAGQAGPQG GNNGGAQDGE
     FHKVDPNK