ID   DNAK_LACLA              Reviewed;         607 AA.
AC   P0A3J0; P42368; Q9CGY8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=LL0954; ORFNames=L178206;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LM0230;
RX   PubMed=8181763; DOI=10.1016/0378-1119(94)90360-3;
RA   Barril M.J.S., Kim S.G., Batt C.A.;
RT   "Cloning and sequencing of the Lactococcus lactis subsp. lactis dnaK gene
RT   using a PCR-based approach.";
RL   Gene 142:91-96(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; X75428; CAA53179.1; -; Genomic_DNA.
DR   EMBL; AE005176; AAK05052.1; -; Genomic_DNA.
DR   PIR; B86744; B86744.
DR   PIR; S39342; S39342.
DR   RefSeq; NP_267110.1; NC_002662.1.
DR   RefSeq; WP_010905652.1; NC_002662.1.
DR   AlphaFoldDB; P0A3J0; -.
DR   SMR; P0A3J0; -.
DR   STRING; 272623.L178206; -.
DR   MoonProt; P0A3J0; -.
DR   PaxDb; P0A3J0; -.
DR   EnsemblBacteria; AAK05052; AAK05052; L178206.
DR   KEGG; lla:L178206; -.
DR   PATRIC; fig|272623.7.peg.1021; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_9; -.
DR   OMA; ISIKRHM; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0009986; C:cell surface; IDA:CAFA.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:2001065; F:mannan binding; IDA:CAFA.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   GO; GO:0044406; P:adhesion of symbiont to host; IDA:CAFA.
DR   GO; GO:0098630; P:aggregation of unicellular organisms; IDA:CAFA.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..607
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078473"
FT   REGION          578..607
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         173
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   VARIANT         103
FT                   /note="A -> S (in strain: LM0230)"
FT   VARIANT         223
FT                   /note="A -> G (in strain: LM0230)"
FT   VARIANT         537
FT                   /note="E -> D (in strain: LM0230)"
FT   VARIANT         584..585
FT                   /note="GQ -> SE (in strain: LM0230)"
FT   VARIANT         592
FT                   /note="A -> G (in strain: LM0230)"
FT   VARIANT         596
FT                   /note="D -> A (in strain: LM0230)"
SQ   SEQUENCE   607 AA;  64987 MW;  4CCF75695368093D CRC64;
     MSKIIGIDLG TTNSAVAVLE GTESKIIPNP EGNRTTPSVV AFKNGEIIVG DAAKRQAVTN
     PETIISIKSK MGTSEKVSAN GKEYTPQEIS AMILQNLKAT AEAYLGEKVE KAVITVPAYF
     NDAQRQATKD AGKIAGLEVE RIVNEPTAAA LAYGLDKTDK DEKILVFDLG GGTFDVSILE
     LGDGVFDVLA TAGNNKLGGD DFDQKIIDWM VAEFKKENGI DLAQDKMALQ RLKDAAEKAK
     KDLSGVTTTQ ISLPFITAGA AGPLHLEMAL TRAKFDELTH DLVEATRQPV RQALSDAGLS
     TSDIDEVLLV GGSTRIPAVV ELVRHETNKE PNKSVNPDEV VAMGAAIQGG VITGDVKDVV
     LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQVFSTAA DNQPAVDIHV LQGERPMAAD
     NKTLGRFQLT DIPAAPRGIP QIEVTFDIDK NGIVSVKAKD LGTQKEQTIV IKSNSGLSDE
     EIDKMMKDAE ANADADAKRK EEVDTRNEAD ALVFQTEKTL KDLEGKVEEA EVKKAEEAKE
     ELKKALEGED IDDIKAKSEA LSEIAQNLAV KLYEQANAAQ GEAGQATDAQ EAPKDDNTFD
     GDFEESK