ID   DNAK_LACLM              Reviewed;         607 AA.
AC   P0A3J1; A2RLI1; P42368; Q9CGY8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=llmg_1574;
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8126443; DOI=10.1099/00221287-139-12-3253;
RA   Eaton T.J., Shearman C.A., Gasson M.J.;
RT   "Cloning and sequence analysis of the dnaK gene region of Lactococcus
RT   lactis subsp. lactis.";
RL   J. Gen. Microbiol. 139:3253-3263(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; X76642; CAA54089.1; -; Genomic_DNA.
DR   EMBL; AM406671; CAL98149.1; -; Genomic_DNA.
DR   PIR; S39342; S39342.
DR   RefSeq; WP_011835408.1; NZ_WJVF01000018.1.
DR   AlphaFoldDB; P0A3J1; -.
DR   SMR; P0A3J1; -.
DR   STRING; 416870.llmg_1574; -.
DR   EnsemblBacteria; CAL98149; CAL98149; llmg_1574.
DR   KEGG; llm:llmg_1574; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_9; -.
DR   OMA; ISIKRHM; -.
DR   PhylomeDB; P0A3J1; -.
DR   BioCyc; LLAC416870:LLMG_RS07930-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..607
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078474"
FT   REGION          577..607
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         173
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   607 AA;  64948 MW;  567FA7160313310E CRC64;
     MSKIIGIDLG TTNSAVAVLE GTESKIIPNP EGNRTTPSVV AFKNGEIIVG DAAKRQAVTN
     PETIISIKSK MGTSEKVSAN GKEYTPQEIS AMILQNLKAT AESYLGEKVE KAVITVPAYF
     NDAQRQATKD AGKIAGLEVE RIVNEPTAAA LAYGLDKTDK DEKILVFDLG GGTFDVSILE
     LGDGVFDVLA TAGNNKLGGD DFDQKIIDWM VAEFKKENGI DLGQDKMALQ RLKDAAEKAK
     KDLSGVTTTQ ISLPFITAGA AGPLHLEMAL TRAKFDELTH DLVEATRQPV RQALSDAGLS
     TSDIDEVLLV GGSTRIPAVV ELVRHETNKE PNKSVNPDEV VAMGAAIQGG VITGDVKDVV
     LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQVFSTAA DNQPAVDIHV LQGERPMAAD
     NKTLGRFQLT DIPAAPRGIP QIEVTFDIDK NGIVSVKAKD LGTQKEQTIV IKSNSGLSDE
     EIDKMMKDAE ANADADAKRK EEVDTRNEAD ALVFQTEKTL KDLEGKVEEA EVKKAEDAKE
     ELKKALEGED IDDIKAKSEA LSEIAQNLAV KLYEQANAAQ GEASEATDAQ EGPKDANTFD
     GDFEESK