ID   DNAK_LATSS              Reviewed;         613 AA.
AC   Q38W93;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=LCA_1236;
OS   Latilactobacillus sakei subsp. sakei (strain 23K) (Lactobacillus sakei
OS   subsp. sakei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=314315;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=23K;
RX   PubMed=16273110; DOI=10.1038/nbt1160;
RA   Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M.,
RA   Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V.,
RA   Zagorec M.;
RT   "The complete genome sequence of the meat-borne lactic acid bacterium
RT   Lactobacillus sakei 23K.";
RL   Nat. Biotechnol. 23:1527-1533(2005).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR936503; CAI55540.1; -; Genomic_DNA.
DR   RefSeq; WP_011374933.1; NC_007576.1.
DR   AlphaFoldDB; Q38W93; -.
DR   SMR; Q38W93; -.
DR   STRING; 314315.LCA_1236; -.
DR   EnsemblBacteria; CAI55540; CAI55540; LCA_1236.
DR   KEGG; lsa:LCA_1236; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_9; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   BioCyc; LSAK314315:LCA_RS06150-MON; -.
DR   Proteomes; UP000002707; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..613
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000225972"
FT   REGION          577..613
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        595..613
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         174
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   613 AA;  66290 MW;  9D920D1E612C0627 CRC64;
     MSKVIGIDLG TTNSAVAVLE GGQPKIITNP EGARTTPSVV SFKNGEIQVG EVAKRQAITN
     PDTIASIKRH IGEAGYKVTV GDKSYTPQEV SAMILQYIKK FAEDYLGEEV TEAVITVPAY
     FNDSQRQATK DAGKIAGLDV KRIINEPTAS ALAYGLDKTE TDEKVLVYDL GGGTFDVSVL
     ELGDGVFQVL STNGDTRLGG DDFDEAIMNW LVENFKSDNG IDLSKDKMAM QRLKDAAEKA
     KKDLSGVTST QISLPFISAG ENGPLHLEMT LSRTEFDRLT SDLVDRTKAP VMNALKDAGL
     DANEIDKVIL NGGSTRIPAV QEAVKNWTGK EPDHSINPDE AVALGAAVQG GVISGDVKDV
     VLLDVTPLSL GIETMGGVFT KLIDRNTTIP TSKAQTFSTA ADNQPAVDIH VLQGERPMAA
     DNKTLGRFQL TDIPAAPRGV PQIEVKFDID KNGIVNVSAK DLGTNKEQKI TIKSNSGLSD
     EEIDRMMKEA QENEEADTKR KEEVDLKNDV DQLIFQTDKT LKELEGKVSD EELQKAKDAK
     EELVKAQQEN NLEDMKTKRD ALSEIVQELT VKLYQQAQEA QQAAGGAEGN ATDAKTDDGT
     VDGDFEEVKD DKE