ID   DNAK_METAC              Reviewed;         617 AA.
AC   Q8TQR2;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; Synonyms=hsp70;
GN   OrderedLocusNames=MA_1478;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; AE010299; AAM04892.1; -; Genomic_DNA.
DR   RefSeq; WP_011021492.1; NC_003552.1.
DR   AlphaFoldDB; Q8TQR2; -.
DR   SMR; Q8TQR2; -.
DR   STRING; 188937.MA_1478; -.
DR   EnsemblBacteria; AAM04892; AAM04892; MA_1478.
DR   GeneID; 1473366; -.
DR   KEGG; mac:MA_1478; -.
DR   HOGENOM; CLU_005965_2_4_2; -.
DR   InParanoid; Q8TQR2; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 10764at2157; -.
DR   PhylomeDB; Q8TQR2; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..617
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078597"
FT   REGION          579..617
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        600..617
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   617 AA;  66209 MW;  95B3C92E418C1676 CRC64;
     MAKILGIDLG TTNSCMAVME GGDAVVLPNA EGSRTTPSVV GFSKKGEKLV GQVAKRQAIS
     NPENTVYSIK RHMGEANYKV TLNGKTYTPQ EISAMILQKL KTEAEAYLGE TIKQAVITVP
     AYFNDAQRQA TKDAGSIAGL EVLRIINEPT AASLAYGLDK GDVDQKILVY DLGGGTFDVS
     ILELGGGVFE VKSTSGDTHL GGDDFDQRVI DHLLAEFKKT EGIDLSKDKA ILQRLKDAAE
     KAKIELSGVT VTNINLPFLT VGPDGEPKHM DIDLTRAQFQ KMTEDLLEKT LVSMRRALSD
     SKLTPNDLDK VILVGGATRM PAVVELVENF TGKKPYKNIN PDEAVAIGAA IQAGVLGGEV
     KDVLLLDVTP LTLGIETLGG IATPLIQRNT TIPTKKSQIF STAADNQPSV EIHVLQGERG
     IASENKTLGR FTLDGIPPAP RGIPQIEVAF DIDANGILHV NAKDLGTGKE QSISIQKPGG
     LSDAEIERMV KDAELHAEED KKRKEEVETR NNAEALINAA EKTIKEAGDV ATEDQKSKVN
     AAIEDLKKAL EGKDTEEVKA KTEALQEAVY PISTAMYQKA QQEAQQASGE AGSADARGPD
     ETVVDADYEV VDDEKRK