ID   DNAK_METI4              Reviewed;         641 AA.
AC   B3DZP7;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=Minf_2178;
OS   Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain
OS   V4)).
OC   Bacteria; Verrucomicrobia; Methylacidiphilae; Methylacidiphilales;
OC   Methylacidiphilaceae; Methylacidiphilum.
OX   NCBI_TaxID=481448;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate V4;
RX   PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA   Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA   Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V.,
RA   Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.,
RA   Feng L., Wang L., Alam M.;
RT   "Complete genome sequence of the extremely acidophilic methanotroph isolate
RT   V4, Methylacidiphilum infernorum, a representative of the bacterial phylum
RT   Verrucomicrobia.";
RL   Biol. Direct 3:26-26(2008).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000975; ACD84232.1; -; Genomic_DNA.
DR   RefSeq; WP_012464514.1; NC_010794.1.
DR   AlphaFoldDB; B3DZP7; -.
DR   SMR; B3DZP7; -.
DR   STRING; 481448.Minf_2178; -.
DR   PRIDE; B3DZP7; -.
DR   EnsemblBacteria; ACD84232; ACD84232; Minf_2178.
DR   KEGG; min:Minf_2178; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_0; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   Proteomes; UP000009149; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..641
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000119728"
FT   REGION          602..641
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        626..641
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         200
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   641 AA;  69708 MW;  9FB7447AD58424DD CRC64;
     MAHVLGIDLG TTNSCMAIVE GGQPVVLENS EGARTTPSIV AFTKSGERLV GQAAKRQAIT
     NSKNTIYSIK RFIGRKFSEV QEEIKRVPYK VIEGKNGDCA VEVEVAGERR VYTPQEISAF
     ILMKLKADAE SKLGEKITQA VITVPAYFND SQRQATKAAG EIAGLEVLRI INEPTAASLA
     YGLDKKKDER IAVYDLGGGT FDISVLEIGE GVFEVKATNG DTHLGGDDWD AAIMEWIISD
     FKKETGIDLH GQPDAVQRIK EEAEKAKIAL SSALEYEINL PFITADQTGP KHIQKKLTRA
     KLEQLTEHLA ERTVQPVLNC LKDAKLTAAD IDELVLVGGM TRMPKIIEIA RKLIGREPHK
     GVNPDEVVAV GAAIQGAVLK GQVKDVLLLD VTPLTLSIET AGGIATPMIP RNTTIPTRKS
     QIFSTFSDNQ PSVEIKVLQG ERPMARDNKL LGVFHLDGIP PAPRGVPQIE VTFDIDANGI
     LHVSAKDLGT GREQKITITG SSGLSKEEIE RMTKEAEAYR EQDLKNKERA EAKNNADNAA
     YQAEKLLREY GDKVDSAKKM EVEKCIEKVR EVIKGDDTEA IKRAMDELNE KVQAISVEMY
     RAASSKASAA SSPPPPPGAG GQKSDVIDAE FEKVDKDKPQ A