ID   DNAK_METPP              Reviewed;         653 AA.
AC   A2SIR4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=Mpe_A2498;
OS   Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Methylibium.
OX   NCBI_TaxID=420662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1232 / LMG 22953 / PM1;
RX   PubMed=17158667; DOI=10.1128/jb.01259-06;
RA   Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W.,
RA   Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M.,
RA   Hristova K.R.;
RT   "Whole-genome analysis of the methyl tert-butyl ether-degrading beta-
RT   proteobacterium Methylibium petroleiphilum PM1.";
RL   J. Bacteriol. 189:1931-1945(2007).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000555; ABM95453.1; -; Genomic_DNA.
DR   RefSeq; WP_011830086.1; NC_008825.1.
DR   AlphaFoldDB; A2SIR4; -.
DR   SMR; A2SIR4; -.
DR   STRING; 420662.Mpe_A2498; -.
DR   PRIDE; A2SIR4; -.
DR   EnsemblBacteria; ABM95453; ABM95453; Mpe_A2498.
DR   KEGG; mpt:Mpe_A2498; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_4; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   Proteomes; UP000000366; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..653
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000059602"
FT   REGION          613..653
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         203
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   653 AA;  69995 MW;  BB5241CCDCB7C0ED CRC64;
     MAKIIGIDLG TTNSCVAIME GNTTKVIENS EGARTTPSII AYQEDGEVLV GASAKRQAVT
     NPRNTLYAVK RLIGRKFTEK EVQKDIDLMP YTIAAAENGD AWVEVRGNKL APPQVSAEVL
     RKMKKTAEDY LGETVTEAVI TVPAYFNDAQ RQATKDAGRI AGLDVKRIIN EPTAAALAFG
     LDKHNDGKGG DRKIAVYDLG GGTFDISIIE IADVDGEMQF EVLSTNGDTF LGGEDFDQRV
     IDYIIAEFKK EQGVDLSKDV LALQRLKEAA EKAKIELSNS TQTDINLPYV TADASGPKHL
     NIKLTRAKLE ALVEELIERT IAPCRTAIKD AGVSTSQIND VILVGGMTRM PKVQEKVKEF
     FGQEPRKDVN PDEAVAVGAA IQGQVLAGDR KDVLLLDVTP LSLGIETLGG VMTKMITKNT
     TIPTKFAQTF STADDNQPAV TIKVFQGERE LASGNKMLGE FNLEGIAPAP RGMPQIEVSF
     DIDANGILHV GAKDKGTGKE NKITIKANSG LSEEEIQKMV KDAELNAAED KKKLELVQAR
     NQADAMVHSV KKSLAEHGDK LDAGEKEKIE SALKDAETAL KGEDKAEIEA KTEALMSASQ
     KLGEKVYADM QAAQAAAGAA AGGGEAPAAE ATASKPADDN VVDAEFKEVK DQK