DNAK_MYCGE
ID DNAK_MYCGE Reviewed; 595 AA.
AC P47547; Q49321;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Chaperone protein DnaK;
DE AltName: Full=HSP70;
DE AltName: Full=Heat shock 70 kDa protein;
DE AltName: Full=Heat shock protein 70;
GN Name=dnaK; Synonyms=hsp70; OrderedLocusNames=MG305;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 353-460.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC -!- INDUCTION: By stress conditions e.g. heat shock (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L43967; AAC71527.1; -; Genomic_DNA.
DR EMBL; U02204; AAD12493.1; -; Genomic_DNA.
DR PIR; G64233; G64233.
DR RefSeq; WP_009885883.1; NZ_AAGX01000008.1.
DR PDB; 5OBU; X-ray; 2.00 A; A=1-521.
DR PDB; 5OBV; X-ray; 2.49 A; A=1-521.
DR PDB; 5OBW; X-ray; 1.40 A; A=1-366.
DR PDB; 5OBX; X-ray; 1.78 A; A=1-366.
DR PDB; 5OBY; X-ray; 1.30 A; A=1-366.
DR PDBsum; 5OBU; -.
DR PDBsum; 5OBV; -.
DR PDBsum; 5OBW; -.
DR PDBsum; 5OBX; -.
DR PDBsum; 5OBY; -.
DR AlphaFoldDB; P47547; -.
DR SMR; P47547; -.
DR STRING; 243273.MG_305; -.
DR PRIDE; P47547; -.
DR EnsemblBacteria; AAC71527; AAC71527; MG_305.
DR KEGG; mge:MG_305; -.
DR eggNOG; COG0443; Bacteria.
DR HOGENOM; CLU_005965_2_1_14; -.
DR OMA; ISIKRHM; -.
DR OrthoDB; 161217at2; -.
DR BioCyc; MGEN243273:G1GJ2-374-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR Gene3D; 2.60.34.10; -; 1.
DR HAMAP; MF_00332; DnaK; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR012725; Chaperone_DnaK.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 1.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Stress response.
FT CHAIN 1..595
FT /note="Chaperone protein DnaK"
FT /id="PRO_0000078490"
FT MOD_RES 182
FT /note="Phosphothreonine; by autocatalysis"
FT /evidence="ECO:0000250"
FT CONFLICT 447..460
FT /note="PKGKPQIEITFSLD -> LKVNPKLRLPLVWM (in Ref. 2)"
FT /evidence="ECO:0000305"
FT STRAND 9..13
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 15..24
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 38..42
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 55..58
FT /evidence="ECO:0007829|PDB:5OBY"
FT TURN 59..63
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:5OBX"
FT HELIX 71..74
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 95..114
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 120..125
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 131..143
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 147..153
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 154..161
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 164..166
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 167..169
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 171..178
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 183..191
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 194..203
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 208..226
FT /evidence="ECO:0007829|PDB:5OBY"
FT TURN 227..229
FT /evidence="ECO:0007829|PDB:5OBX"
FT HELIX 232..234
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 236..255
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 256..269
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 272..281
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 282..288
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 290..295
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 297..307
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 311..313
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 316..321
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 322..325
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 327..336
FT /evidence="ECO:0007829|PDB:5OBY"
FT TURN 348..350
FT /evidence="ECO:0007829|PDB:5OBY"
FT HELIX 351..364
FT /evidence="ECO:0007829|PDB:5OBY"
FT STRAND 380..384
FT /evidence="ECO:0007829|PDB:5OBU"
FT TURN 385..387
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 388..393
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 398..411
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 417..425
FT /evidence="ECO:0007829|PDB:5OBU"
FT HELIX 429..431
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 432..440
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 453..459
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 465..471
FT /evidence="ECO:0007829|PDB:5OBU"
FT TURN 472..474
FT /evidence="ECO:0007829|PDB:5OBU"
FT STRAND 477..482
FT /evidence="ECO:0007829|PDB:5OBU"
FT HELIX 490..502
FT /evidence="ECO:0007829|PDB:5OBU"
FT HELIX 504..511
FT /evidence="ECO:0007829|PDB:5OBU"
SQ SEQUENCE 595 AA; 65051 MW; 8EB48D058A009FB7 CRC64;
MSADNGLIIG IDLGTTNSCV SVMEGGRPVV LENPEGKRTT PSIVSYKNNE IIVGDAAKRQ
MVTNPNTIVS IKRLMGTSNK VKVQNADGTT KELSPEQVSA QILSYLKDFA EKKIGKKISR
AVITVPAYFN DAERNATKTA GKIAGLNVER IINEPTAAAL AYGIDKASRE MKVLVYDLGG
GTFDVSLLDI AEGTFEVLAT AGDNRLGGDD WDNKIIEYIS AYIAKEHQGL NLSKDKMAMQ
RLKEAAERAK IELSAQLETI ISLPFLTVTQ KGPVNVELKL TRAKFEELTK PLLERTRNPI
SDVIKEAKIK PEEINEILLV GGSTRMPAVQ KLVESMVPGK KPNRSINPDE VVAIGAAIQG
GVLRGDVKDV LLLDVTPLTL SIETLGGVAT PLIKRNTTIP VSKSQIFSTA QDNQESVDVV
VCQGERPMSR DNKSLGRFNL GGIQPAPKGK PQIEITFSLD ANGILNVKAK DLTTQKENSI
TISDNGNLSE EEIQKMIRDA EANKERDNII RERIELRNEG EGIVNTIKEI LASPDAKNFP
KEEKEKLEKL TGNIDAAIKA NDYAKLKVEI ENFKKWREEM AKKYNPTGEQ GPQAK
