ID   DNAK_MYCPA              Reviewed;         623 AA.
AC   Q00488; Q9L589;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=70 kDa antigen;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=MAP_3840;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1886778; DOI=10.1093/nar/19.16.4552;
RA   Stevenson K., Inglis N.F., Rae B., Donachie W., Sharp J.M.;
RT   "Complete nucleotide sequence of a gene encoding the 70 kd heat shock
RT   protein of Mycobacterium paratuberculosis.";
RL   Nucleic Acids Res. 19:4552-4552(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Koets A.P., Rutten V.P.M.G., de Boer M., Bakker D., Valentin-Weigand P.,
RA   van Eden W.;
RT   "Differential changes in heat shock protein, lipoarabinomannan and PPD
RT   specific immunoglobulin G1 and G2 isotype responses during bovine
RT   Mycobacterium avium ssp. paratuberculosis infection.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; X59437; CAA42063.1; -; Genomic_DNA.
DR   EMBL; AF254578; AAF65842.1; -; Genomic_DNA.
DR   EMBL; AE016958; AAS06390.1; -; Genomic_DNA.
DR   PIR; S34440; S34440.
DR   RefSeq; WP_003879232.1; NC_002944.2.
DR   AlphaFoldDB; Q00488; -.
DR   SMR; Q00488; -.
DR   STRING; 262316.MAP_3840; -.
DR   EnsemblBacteria; AAS06390; AAS06390; MAP_3840.
DR   KEGG; mpa:MAP_3840; -.
DR   PATRIC; fig|262316.17.peg.4087; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_11; -.
DR   OMA; ISIKRHM; -.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 2.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..623
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078494"
FT   REGION          587..623
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         175
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        8..9
FT                   /note="DL -> RY (in Ref. 1; CAA42063)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   623 AA;  66518 MW;  02EB979BD413AAF6 CRC64;
     MARAVGIDLG TTNSVVAVLE GGDPVVVANS EGSRTTPSIV AFARNGEVLV GQPAKNQAVT
     NVDRTIRSVK RHMGTDWSIE IDGKKYTAQE ISARVLMKLK RDAEAYLGED ITDAVITVPA
     YFNDAQRQAT KEAGQIAGLN VLRIVNEPTA AALAYGLDKG EKEQTILVFD LGGGTFDVSL
     LEIGEGVVEV RATSGDNQLG GDDWDDRIVN WLVDKFKGTS GIDLTKDKMA MQRLREAAEK
     AKIELSSSQS TSINLPYITV DADKNPLFLD EQLTRAEFQR ITQDLLDRTR QPFKSVIADA
     GISVSDIDHV VLVGGSTRMP AVTDLVKELT GGKEPNKGVN PDEVVAVGAA LQAGVLKGEV
     KDVLLLDVTP LSLGIETKGG VMTKLIERNT TIPTKRSETF TTADDNQPSV QIQVYQGERE
     IAAHNKLLGS FELTGIPPAP RGVPQIEVTF DIDANGIVHV TAKDKGTGKE NTIKIQEGSG
     LSKEEIDRMI KDAEAHAEED RKRREEADVR NQAESLVYQT EKFVKDQREA EGGSKVPEET
     LSKVDAAIAD AKTALGGTDI TAIKSAMEKL GQESQALGQA IYEATQAESA QAGGPDGAAA
     GGGSGSADDV VDAEVVDDDR ESK