ADDB_LYSSC
ID ADDB_LYSSC Reviewed; 1169 AA.
AC B1HN89;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=Bsph_1198;
OS Lysinibacillus sphaericus (strain C3-41).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX NCBI_TaxID=444177;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C3-41;
RX PubMed=18296527; DOI=10.1128/jb.01652-07;
RA Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M.,
RA Berry C., Yuan Z.;
RT "Complete genome sequence of the mosquitocidal bacterium Bacillus
RT sphaericus C3-41 and comparison with those of closely related Bacillus
RT species.";
RL J. Bacteriol. 190:2892-2902(2008).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
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DR EMBL; CP000817; ACA38806.1; -; Genomic_DNA.
DR RefSeq; WP_012292937.1; NC_010382.1.
DR AlphaFoldDB; B1HN89; -.
DR SMR; B1HN89; -.
DR PRIDE; B1HN89; -.
DR EnsemblBacteria; ACA38806; ACA38806; Bsph_1198.
DR KEGG; lsp:Bsph_1198; -.
DR HOGENOM; CLU_007838_0_0_9; -.
DR OMA; DRLENYV; -.
DR Proteomes; UP000002164; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding.
FT CHAIN 1..1169
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379199"
FT DOMAIN 1..296
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT DOMAIN 276..582
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 8..15
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 804
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1129
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1132
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1138
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1169 AA; 135538 MW; 04066AF30A7B39BB CRC64;
MTLRIVSGRS GTGKSVFIHQ EIVEQLKSDP LGHPIFIIVP DQMSYSTEYE LTNRHGLQGL
IRAQVMTFKR LAWLVLQETG GIARKEVNGY GYRMLIRKLL EEQKSEFSLF RQAAGKRGFT
EEIETLLKEF SRYSVNSSVL AEVKESLKAI DAPNTLQAKT NDLYVVLQAL EERLGTTYVD
SEGYYPILTE QLKYAETMKQ ATIYIDGFTA FTVRELELVR ELLKVTKHVT VVLPFDHIDE
AFDEQALFHE AALTNQRLHD IANEEGIDVE PPLHFYYTQR FQSEDLQHVE ANFANMVPHT
KKTSGDVMVF EASNRRAEVH AIAREITKLT REYGYRYQDI VLLYRQAELY DPLITSIFQQ
YEIPIFTNTK KTMLHHPLIE LSRSALEIMT SNWKYEPVFR SVKTDLFFPL QAELTIWRER
ADRLENYCLA QGIYGERWFE EPRWFYKKYR GLEFHSRVQT DEERAMQAEI EAIRDEIRQP
LKSLQDKLSI ASTGKDIATA LFELVESLQV YEKLQAMKDR ELERGDALAA SEHEQAWNEW
INVLDQFVYM FGEQEMSVEE AAKILDEGFD TLEFSRIPPT LDEVMVATVD LARLSNIKVA
FVLGMNDGVY PTRMEYEGLL SDTEREWFSQ IGYELAPTSS NRLLQENFLF YRAASTPSNK
LYLTYPTADE EGKALLSSLY IKKFIGNDKI AGLLSGVQAE RVVMDPIELL DESALPYLRH
PRTALAHLMV QLRQAEHSRE LAPEWLALQK FYQQDPYWAL IFDRVHYPIT HKNEAEPLET
YITQELYGQK LTSSVSRIEK YFRCPFSHFT TYGLRLEERA EYRLETFAMG DLFHEALKWI
TEETHRLQLS WIRLTKQQIK QLARQAVEQI VPVFSHQILL SSARYRYIQR KLIRIVERTM
MALTQHANVS HFKPIAIEAS FGPGQHEQLP PLEIDLTSGK KMFMRGRIDR IDSATIDDRS
YLRIVDYKSS ARDLDLNEVY YGLSLQVLTY LDVAMENSTY WLPGETEPAG VLYVHVHNPM
LKLDKDMTDS EIEEDRLKQY KMKGLLSENA ESILSMDEQL EESSGHSKII PVYMKKDGTP
SESQSRIVPV NDMKRLQHFV RRKHQEAGNG ILSGDTAISP YKLKSKTACD YCQFAAVCQF
DPSDGKQNYR QLMQAKPNEI VDKIRKEIE
