ID   DNAK_NITEU              Reviewed;         644 AA.
AC   O06430;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=NE1949;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=9143112; DOI=10.1128/aem.63.5.1777-1784.1997;
RA   Iizumi T., Nakamura K.;
RT   "Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas
RT   europaea dnaK gene.";
RL   Appl. Environ. Microbiol. 63:1777-1784(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR   EMBL; AB018706; BAA33935.1; -; Genomic_DNA.
DR   EMBL; AL954747; CAD85860.1; -; Genomic_DNA.
DR   RefSeq; WP_011112481.1; NC_004757.1.
DR   AlphaFoldDB; O06430; -.
DR   SMR; O06430; -.
DR   STRING; 228410.NE1949; -.
DR   EnsemblBacteria; CAD85860; CAD85860; NE1949.
DR   KEGG; neu:NE1949; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_4; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   PhylomeDB; O06430; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..644
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078504"
FT   REGION          597..644
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        606..625
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        627..644
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         199
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   644 AA;  69695 MW;  8E8EF50CE3E5D71C CRC64;
     MAKIIGIDLG TTNSCVAVME GNKPKVIENA EGARTTPSIV AYAEDNEILV GASAKRQAVT
     NPENTLFAIK RLIGRRFDEE VVQKDISVTP YKIVRADNND AWIEARGRKI APPEVSAQVL
     IKMKKTAEDY LGEPVTEAVI TVPAYFNDSQ RQATKDAGRI AGLEVKRIIN EPTAAALAFG
     LDKKEGDRKI AVYDLGGGTF DISIIEIAEV EGEHQFEVLA TNGDTFLGGE DFDSRVIEYL
     VDEFRKESGI DLKKDMLALQ RLKDAAEKAK IELSSSQQTE VNLPYITADA SGPKHLAVKI
     TRAKLESLVE ELIERTAGPC RTALKDAGLS VSDINDVILV GGQTRMPKVQ EKVKEIFGKE
     PRKDVNPDEA VAIGAAIQGG VLKGDVKDVL LLDVTPLSLG IETLGGVMTK LIQKNTTIPT
     KAQQVFSTAD DNQTAVTIHV LQGEREVASG NKSLGQFNLT DIPSAPRGMP QIEVIFDIDA
     NGILHVSAKD KATGKENKIK IQASSGLSED EIQKMVKDAE AHAEEDKKAL DLVNSRNQCD
     AMIHSVRKSL AEYGDKLEGD EKSRIEAALK EAEEALKSGD KQTIDAKTQA LTEASHKLAE
     KMYAQEQAQA GQQAGAGTAS DQSQDKPVEG EVVDAEFEEV KDKK