ID   DNAK_NOCSJ              Reviewed;         621 AA.
AC   A1SPX5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=Noca_4363;
OS   Nocardioides sp. (strain ATCC BAA-499 / JS614).
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Nocardioides.
OX   NCBI_TaxID=196162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-499 / JS614;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J.,
RA   Richardson P.;
RT   "Complete sequence of chromosome 1 of Nocardioides sp. JS614.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000509; ABL83860.1; -; Genomic_DNA.
DR   RefSeq; WP_011757789.1; NC_008699.1.
DR   AlphaFoldDB; A1SPX5; -.
DR   SMR; A1SPX5; -.
DR   STRING; 196162.Noca_4363; -.
DR   PRIDE; A1SPX5; -.
DR   EnsemblBacteria; ABL83860; ABL83860; Noca_4363.
DR   KEGG; nca:Noca_4363; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_11; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   Proteomes; UP000000640; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..621
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000059620"
FT   REGION          580..621
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        598..613
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         174
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   621 AA;  66109 MW;  F6AC9C0B0E066F4D CRC64;
     MARAVGIDLG TTNSVVAVLE GGEPTVIANA EGARTTPSVV AFAKSGEVLV GEVAKRQAVT
     NVDRTIRSVK RHMGTDWLTK IDDKDFTPQQ ISAFVLQKLK RDAEAYLGEP VTDAVITVPA
     YFSDAQRQAT KEAGEIAGLN VSRIVNEPTA AALAYGLDKG DDQTILVFDL GGGTFDVSLL
     EIGEGVVEVK ATSGDNHLGG DDWDARIVDW MVKKFKDNNG VDLAADKIAK QRLQEAAEKA
     KIELSSSSET TIHLPYITHG ESGPLHFEEK LTRSEFQRLT TDLLDRTKGP FQSVLKDGGV
     AIKDIDHVVL VGGSTRMPAV TEVVKELLGG KEPNKGVNPD EVVAVGAALQ AGVLKGEVKD
     VLLLDVTPLS LGIETKGGVM TTLIERNTTI PTKRSEIFTT ADDNQPSVEI KVAQGERQMW
     AQNQPLGNFE LTGLPPAPRG IPKIEVTFDI DANGIVHVTA KDQASGKEQS MTISGGSALG
     KDEIDRMVRE AEQYAEEDAK RREAVETRNQ AEQLVYTTEK FLDENSDKLP DDVKTEVRAD
     VDALKVTLEK EDASADDIRA GVTKLGESSQ KMGAAMYAAA EADSAAAGGS AGATGESDDD
     VVDAEIVDEG GADDGAEGES K