ID   DNAK_VARPS              Reviewed;         645 AA.
AC   C5CU12;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=Vapar_1712;
OS   Variovorax paradoxus (strain S110).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Variovorax.
OX   NCBI_TaxID=543728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S110;
RX   PubMed=21183664; DOI=10.1128/jb.00925-10;
RA   Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G.,
RA   O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C.,
RA   Ovchinnikova G., Goodwin L., Han C.;
RT   "Complete genome sequence of the metabolically versatile plant growth-
RT   promoting endophyte, Variovorax paradoxus S110.";
RL   J. Bacteriol. 193:1183-1190(2011).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP001635; ACS18362.1; -; Genomic_DNA.
DR   RefSeq; WP_012746854.1; NC_012791.1.
DR   AlphaFoldDB; C5CU12; -.
DR   SMR; C5CU12; -.
DR   STRING; 543728.Vapar_1712; -.
DR   EnsemblBacteria; ACS18362; ACS18362; Vapar_1712.
DR   GeneID; 45056018; -.
DR   KEGG; vap:Vapar_1712; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_4; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..645
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000205201"
FT   REGION          608..645
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         200
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   645 AA;  69013 MW;  B5F01127E85D18AA CRC64;
     MAKIIGIDLG TTNSCVSIME GNTTRVIENS EGARTTPSIV AYQEDGEVLV GASAKRQAVT
     NPKNTLYAIK RLIGRKFEEK EVQKDIDLMP YTIAKADNGD AWVEVRGKKI APQQVSADIL
     RKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLDVKRIIN EPTAAALAFG
     LDKQDKADRK IAVYDLGGGT FDISIIEIAD VDGEKQFEVL STNGDTFLGG EDFDQRIIDY
     IIAEFKKEQG VDLGKDVLAL QRLKEAAEKA KIELSNSAQT DINLPYITAD ASGPKHLNIK
     LTRAKLESLV DELVERTIAP CRLAIKDAGI SVSDINDVIL VGGMTRMPKV QEKVKAFFGK
     EPRKDVNPDE AVAVGAAIQG QVLSGDRKDV LLLDVTPLSL GIETMGGVMT KMITKNTTIP
     TKFAQTFSTA EDNQPAVTIK VFQGEREIAS GNKLLGEFNL EGIPPAARGT PQIEVSFDID
     ANGILHVGAK DKGTGKENKI TIKANSGLSE DEIQKMVKDA ELNAAEDKKK VELAQARNQG
     EAMVHSVKKS LGEHGASLDA GEKEKIEAAI KDLEEALKGE DKASIEEKTN TLMTASQKLG
     EKMYADAQAA AGAAGGPGAA AGPEAASAPA DDNVVDAEVK EVKKG