ID   DNAK_VIBC3              Reviewed;         635 AA.
AC   A5F361; C3LYL9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332};
GN   OrderedLocusNames=VC0395_A0382, VC395_0871;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP000627; ABQ21320.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08885.1; -; Genomic_DNA.
DR   RefSeq; WP_000516147.1; NZ_JAACZH010000023.1.
DR   AlphaFoldDB; A5F361; -.
DR   SMR; A5F361; -.
DR   STRING; 345073.VC395_0871; -.
DR   EnsemblBacteria; ABQ21320; ABQ21320; VC0395_A0382.
DR   GeneID; 57739554; -.
DR   KEGG; vco:VC0395_A0382; -.
DR   KEGG; vcr:VC395_0871; -.
DR   PATRIC; fig|345073.21.peg.844; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_6; -.
DR   OMA; ISIKRHM; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Stress response.
FT   CHAIN           1..635
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000072043"
FT   REGION          601..635
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        601..615
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         198
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   635 AA;  68761 MW;  2A05D6F5411EC953 CRC64;
     MGKIIGIDLG TTNSCVAVLD GDKPRVIENA EGERTTPSVI AYTDGETLVG QPAKRQAVTN
     PQNTLFAIKR LIGRRFEDEE VQRDIKIMPY KIVKADNGDA WVEAKGQKMA APQVSAEVLK
     KMKKTAEDFL GEPVTAAVIT VPAYFNDAQR QATKDAGRIA GLEVKRIINE PTAAALAYGL
     DKQGGDRTIA VYDLGGGTFD ISIIEIDEVE GEKTFEVLST NGDTHLGGED FDNRMINYLV
     DEFKKDQGID LRNDPLAMQR LKEAAEKAKI ELSSAQQTDV NLPYITADAT GPKHMNIKVT
     RAKLEALVED LVQRSLEPLK VALADADLSV NDITDVILVG GQTRMPMVQK KVAEFFGKEP
     RKDVNPDEAV AVGAAVQGGV LAGEVKDVLL LDVTPLSLGI ETMGGVMTKL IEKNTTIPTK
     ANQVFSTAED NQSAVTIHVL QGERKQAMYN KSLGQFNLEG INPAPRGMPQ IEVIFDLDAD
     GILHVSAKDK QTGKEQKITI QASGGLSDAE IEKMVQEAEA NKEADKKFEE LATARNQADQ
     MIHATRKQIT EAGEALPADE KAKIETAINE LETAKKGEDK AEIDAKVQAL MAAAQKLMEI
     AQQQAQAQGA NAGQSSAKED DVVDAEFEEV NDDKK