DND1_DANRE
ID DND1_DANRE Reviewed; 411 AA.
AC Q7T1H5; Q4VBK4;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Dead end protein 1;
GN Name=dnd;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN PGC MIGRATION, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=12932328; DOI=10.1016/s0960-9822(03)00537-2;
RA Weidinger G., Stebler J., Slanchev K., Dumstrei K., Wise C.,
RA Lovell-Badge R., Thisse C., Thisse B., Raz E.;
RT "Dead end, a novel vertebrate germ plasm component, is required for
RT zebrafish primordial germ cell migration and survival.";
RL Curr. Biol. 13:1429-1434(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, MUTAGENESIS OF TYR-104, AND SUBCELLULAR LOCATION.
RX PubMed=18155131; DOI=10.1016/j.cell.2007.11.034;
RA Kedde M., Strasser M.J., Boldajipour B., Oude Vrielink J.A., Slanchev K.,
RA le Sage C., Nagel R., Voorhoeve P.M., van Duijse J., Oerom U.A., Lund A.H.,
RA Perrakis A., Raz E., Agami R.;
RT "RNA-binding protein Dnd1 inhibits microRNA access to target mRNA.";
RL Cell 131:1273-1286(2007).
CC -!- FUNCTION: RNA-binding factor that positively regulates gene expression
CC by prohibiting miRNA-mediated gene suppression. Relieves miRNA
CC repression in germline cells. Prohibits the function of several miRNAs
CC by blocking the accessibility of target mRNAs (By similarity).
CC Sequence-specific RNA-binding factor that binds to U-rich regions
CC (URRs) in the 3'untranslated region (3'-UTR) of several mRNAs (By
CC similarity). Does not bind to miRNAs (By similarity). Germline-specific
CC protein required for the primordial germ cell (PGC) survival and
CC migration during early embryonic development. {ECO:0000250,
CC ECO:0000269|PubMed:12932328, ECO:0000269|PubMed:18155131}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Perinuclear germ
CC granules, also called germ plasm or chromatoid body.
CC -!- DEVELOPMENTAL STAGE: Expressed in the germ plasm and the primordial
CC germ cells (PGCs). At the onset of embryogenesis, maternal product is
CC located in granules distributed throughout the cortex of the one-cell
CC stage embryo. At 0.5 hour post fertilization (hpf) is expressed at the
CC vegetal part of the blastomere. At 1 hpf expressed at the distal parts
CC of the first two cleavage furrows. At 4 hpf expressed exclusively in
CC PGCs. Expressed in PGCs during their migration and at their arrival in
CC the presumptive gonade. At 5 days post-fertilization (dpf) still
CC expressed in PGCs, albeit at a lower level.
CC {ECO:0000269|PubMed:12932328}.
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DR EMBL; AY225448; AAP22373.1; -; mRNA.
DR EMBL; BC095578; AAH95578.1; -; mRNA.
DR RefSeq; NP_997960.1; NM_212795.1.
DR AlphaFoldDB; Q7T1H5; -.
DR SMR; Q7T1H5; -.
DR STRING; 7955.ENSDARP00000035017; -.
DR PaxDb; Q7T1H5; -.
DR Ensembl; ENSDART00000036463; ENSDARP00000035017; ENSDARG00000022813.
DR GeneID; 373074; -.
DR KEGG; dre:373074; -.
DR CTD; 373863; -.
DR ZFIN; ZDB-GENE-030828-4; dnd1.
DR eggNOG; KOG0117; Eukaryota.
DR GeneTree; ENSGT00940000159225; -.
DR HOGENOM; CLU_022960_0_0_1; -.
DR InParanoid; Q7T1H5; -.
DR OMA; QLWCERV; -.
DR OrthoDB; 1384330at2759; -.
DR PhylomeDB; Q7T1H5; -.
DR TreeFam; TF314932; -.
DR PRO; PR:Q7T1H5; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 14.
DR Bgee; ENSDARG00000022813; Expressed in mature ovarian follicle and 23 other tissues.
DR ExpressionAtlas; Q7T1H5; baseline.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0043073; C:germ cell nucleus; IDA:ZFIN.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0043186; C:P granule; IDA:ZFIN.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:ZFIN.
DR GO; GO:0003729; F:mRNA binding; IDA:ZFIN.
DR GO; GO:0003723; F:RNA binding; IPI:ZFIN.
DR GO; GO:0007281; P:germ cell development; IMP:ZFIN.
DR GO; GO:0008354; P:germ cell migration; IMP:ZFIN.
DR GO; GO:0060047; P:heart contraction; IMP:ZFIN.
DR GO; GO:0048255; P:mRNA stabilization; IMP:ZFIN.
DR GO; GO:0060965; P:negative regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ZFIN.
DR GO; GO:2000145; P:regulation of cell motility; IMP:ZFIN.
DR CDD; cd20313; DSRM_DND1; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR044448; DND1_DSRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..411
FT /note="Dead end protein 1"
FT /id="PRO_0000081570"
FT DOMAIN 60..138
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT MUTAGEN 104
FT /note="Y->C: Inhibits RNA-binding and the relieve of miRNA
FT repression activity."
FT /evidence="ECO:0000269|PubMed:18155131"
SQ SEQUENCE 411 AA; 46020 MW; 36CE5A3F12DFFFD1 CRC64;
MVGDMDAQQQ ELQQILNPQK LKSLQEWMQR NSITLTQVNG QRKYGGPPPG WQGPAPGSGC
EVFISQIPND VYEDRLIPLF QSIGTIYEFR LMMNFSGQTR GFAYAKYGDP LTASAAVTTL
HQYRLPEGGC LTVRRSTEKR QLRLGDLPVS MNESKLLMVL QMLSDGVEDV LLKPPGPKGK
EVVALVNYTS HYAASMAKKV LVEAFRNRYG ISITVRWTSF SKSKRVEDTP QEDSCVTPLV
LKPLSKPSLL HYDVPAHQSL LPLFRAVGGP TTSEQRDEMI PQPTIMSRNE LIPQSSIRQR
DEMVPQLPIR PRDGMAPQSP ISLDAVSHLQ WMCEVNRLGS PQYEVHFHHA APDGFLYFAF
KVLIPGLPLP LYGFVQILPG TSARAMKSEV YRAAAEQVIQ TLCRVSNLRP F
