ID   DNE1_CHLAP              Reviewed;         218 AA.
AC   Q32001;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=DNA endonuclease I-ChuI;
DE            EC=3.1.-.-;
OS   Chlamydomonas applanata (Chlamydomonas humicola).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=35704;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8335261; DOI=10.1016/0378-1119(93)90697-2;
RA   Cote V., Mercier J.P., Lemieux C., Turmel M.;
RT   "The single group-I intron in the chloroplast rrnL gene of Chlamydomonas
RT   humicola encodes a site-specific DNA endonuclease (I-ChuI).";
RL   Gene 129:69-76(1993).
CC   -!- FUNCTION: Probable endonuclease involved in intron homing. Encoded in
CC       the group-I intron of the subunit rRNA-encoding gene (rrnL), it
CC       generates a staggered cut with 4-nt (CTCG) 3'-OH overhangs 2 bp
CC       downstream from the intron insertion site.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; L06107; AAA02744.1; -; Genomic_DNA.
DR   PIR; JC1520; JC1520.
DR   AlphaFoldDB; Q32001; -.
DR   SMR; Q32001; -.
DR   REBASE; 2618; I-ChuI.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF03161; LAGLIDADG_2; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Endonuclease; Hydrolase; Intron homing; Nuclease; Plastid.
FT   CHAIN           1..218
FT                   /note="DNA endonuclease I-ChuI"
FT                   /id="PRO_0000192782"
SQ   SEQUENCE   218 AA;  25133 MW;  13DAC763140F855E CRC64;
     MSLTQQQKDL IFGSLLGDGN LQTGSVGRTW RYRALHKSEH QTYLFHKYEI LKPLCGENTL
     PTESIVFDER TNKEVKRWFF NTLTNPSLKF FADMFYTYDQ NTQKWVKDVP VKVQTFLTPQ
     ALAYFYIDDG ALKWLNKSNA MQICTESFSQ GGTIRIQKAL KTLYNIDTTL TKKTLQDGRI
     GYRIAIPEAS SGAFREVIKP FLVDCMRYKV SDGNKGHL