ID   DNE1_CHLVU              Reviewed;         161 AA.
AC   P56347;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=DNA endonuclease I-CvuI;
DE            EC=3.1.-.-;
DE   AltName: Full=23S rRNA intron protein;
OS   Chlorella vulgaris (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX   NCBI_TaxID=3077;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAM C-27 / Tamiya;
RX   PubMed=9159184; DOI=10.1073/pnas.94.11.5967;
RA   Wakasugi T., Nagai T., Kapoor M., Sugita M., Ito M., Ito S., Tsudzuki J.,
RA   Nakashima K., Tsudzuki T., Suzuki Y., Hamada A., Ohta T., Inamura A.,
RA   Yoshinaga K., Sugiura M.;
RT   "Complete nucleotide sequence of the chloroplast genome from the green alga
RT   Chlorella vulgaris: the existence of genes possibly involved in chloroplast
RT   division.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:5967-5972(1997).
CC   -!- FUNCTION: Probable endonuclease involved in intron homing.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000305}.
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DR   EMBL; AB001684; BAA57868.1; -; Genomic_DNA.
DR   PIR; T07221; T07221.
DR   RefSeq; NP_045793.1; NC_001865.1.
DR   PDB; 5A72; X-ray; 2.60 A; A/B=2-161.
DR   PDB; 5A74; X-ray; 2.50 A; A/B=2-161.
DR   PDB; 5A77; X-ray; 2.50 A; A/B=2-161.
DR   PDB; 5A78; X-ray; 2.50 A; A/B=2-161.
DR   PDBsum; 5A72; -.
DR   PDBsum; 5A74; -.
DR   PDBsum; 5A77; -.
DR   PDBsum; 5A78; -.
DR   AlphaFoldDB; P56347; -.
DR   SMR; P56347; -.
DR   GeneID; 809197; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF00961; LAGLIDADG_1; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Endonuclease; Hydrolase; Intron homing;
KW   Nuclease; Plastid.
FT   CHAIN           1..161
FT                   /note="DNA endonuclease I-CvuI"
FT                   /id="PRO_0000192784"
FT   HELIX           6..22
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   STRAND          23..31
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   STRAND          35..50
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           51..54
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           55..64
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   STRAND          68..71
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   STRAND          75..83
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           84..94
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   TURN            95..97
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   STRAND          99..101
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           102..114
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           115..117
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           121..135
FT                   /evidence="ECO:0007829|PDB:5A74"
FT   HELIX           147..156
FT                   /evidence="ECO:0007829|PDB:5A74"
SQ   SEQUENCE   161 AA;  18055 MW;  8A6752241E3EE7A9 CRC64;
     MQPTNFHDQL KFAWLAGFVD ADGCINAQIV SREDYLLKYQ VRVSLTVFQS TTRHFILLDI
     QKILGCGTVR KRNDGMSEFC VVGGTSLQTT LEKLLPYLQL KRAQAKLVLQ IIKKLPNTKD
     PSVLMEAALL ADKVGLLTDG KKRTILAENV RECLKKLGHV V