ID   DNE21_AGRFC             Reviewed;        1091 AA.
AC   Q8UAY9; Q7CRY1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Error-prone DNA polymerase 1 {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2-1 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Atu3228;
GN   ORFNames=AGR_L_3173;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE007870; AAK90161.1; -; Genomic_DNA.
DR   PIR; AF2953; AF2953.
DR   PIR; G98329; G98329.
DR   RefSeq; NP_357376.1; NC_003063.2.
DR   RefSeq; WP_010972863.1; NC_003063.2.
DR   AlphaFoldDB; Q8UAY9; -.
DR   SMR; Q8UAY9; -.
DR   STRING; 176299.Atu3228; -.
DR   PRIDE; Q8UAY9; -.
DR   EnsemblBacteria; AAK90161; AAK90161; Atu3228.
DR   GeneID; 66223540; -.
DR   KEGG; atu:Atu3228; -.
DR   PATRIC; fig|176299.10.peg.3069; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; VAKSHHW; -.
DR   PhylomeDB; Q8UAY9; -.
DR   BioCyc; AGRO:ATU3228-MON; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0009432; P:SOS response; EXP:CollecTF.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1091
FT                   /note="Error-prone DNA polymerase 1"
FT                   /id="PRO_0000103362"
FT   REGION          1051..1080
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1091 AA;  122865 MW;  7E1C7FD9EDB33CB2 CRC64;
     MSAPRYAELQ VTTHFSFLRG ASSCDELFEQ AKNLGIEALG VVDRNSLAAI PRAYEAANNH
     GVRLVIGCRL DLDDDLSVLV YPMDRAAYGR LCRLLSVGKK RGGKGKCRLS WDDLVAYGEG
     LIVVLLADLA DDLCALRLRR LKAAFADRAY MALSLRRRPN DQMRLFELSG MAQAAGVPTV
     VTNDVLFHVP ERRMLQDVVT CIRHNCTIDE AGFRRERHAD RYMKPPEEMH RLFARYPEAL
     SRSLEIAKRC KFSLKELVYQ YPEERSLPGL TAQQALEKMV WEAVPGRYPN GLPEKVEKAL
     HHELDVVGRL QYASYFLTVN AIVRYARSKD ILCQGRGSAA NSVICFVLGI TAIDPALFSN
     LVFERFVSEN RGEPPDIDVD FEHQRREEVI QWVYDTYGRD KAALCSVVTR YRGRGALRDV
     GKVLGLPEDL TKLLSSQVWR WSEGVGEKQV KELNLNMEDR RLKLAFELAN QLVGTPRHHS
     QHPGGFVLSH DRLDELVPIE PAAMNDRQII EWDKDDIDIV KFMKMDCLAL GMLSCMKRGF
     DLLEARTGEK YDLAAMPPDD PATFAMIQKA DTLGTFQIES RAQMSMLPRL KPAKFYDLVI
     QVAIVRPGPI QGDMVHPYLR RRQGKEPVLY EKPQLENILK KTLGVPLFQE QAMRIAMDCA
     DFTADEADQL RRAMATFKNV GTISKFKEKL VTGMVANGYD KEFAERIFKQ LEGFGSYGFP
     ESHAASFALI AYASSWLKCH HPDIFCTAIL NSQPMGFYAP AQIVRDARDH GVEVRPVCVN
     NSRFDCTLEP TGKKNDKGEE RFAVRLGLRM VKGLSNDHAA DIVAARQDRP FASVDDLWRR
     AGVPAAALVC LAEADAFLPS LRLARREALW AIKALRDEPL PLFAAAAIRE NAVIEELQEP
     SVALRPMTDG GEVVQDYGHV GLTLREHPMT FLRRDLSRRR IVTCAEAVRV RDGTWLETAG
     LVLVRQRPGS AKGVIFMTLE DETGIANAVL WVKTFEKYRR VVLSAGMVGI YGKIQREGEV
     VHLVAHRLTD LSHALASVGE RNNAFPLPHG RGDEFHHGMP DDHRAIRKRP PPSNHDDDEV
     ERIKVISRNF H