ID   DNE22_AGRFC             Reviewed;        1085 AA.
AC   Q8UKK2; Q7D3X1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Error-prone DNA polymerase 2 {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2-2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Atu5100;
GN   ORFNames=AGR_pAT_bx5;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid AT.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE007872; AAK90476.1; -; Genomic_DNA.
DR   PIR; AH3171; AH3171.
DR   RefSeq; NP_396035.1; NC_003064.2.
DR   RefSeq; WP_010974364.1; NC_003064.2.
DR   AlphaFoldDB; Q8UKK2; -.
DR   SMR; Q8UKK2; -.
DR   STRING; 176299.Atu5100; -.
DR   PRIDE; Q8UKK2; -.
DR   EnsemblBacteria; AAK90476; AAK90476; Atu5100.
DR   KEGG; atu:Atu5100; -.
DR   PATRIC; fig|176299.10.peg.4793; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; HTNSYYN; -.
DR   PhylomeDB; Q8UKK2; -.
DR   BioCyc; AGRO:ATU5100-MON; -.
DR   Proteomes; UP000000813; Plasmid At.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1085
FT                   /note="Error-prone DNA polymerase 2"
FT                   /id="PRO_0000103363"
FT   REGION          1040..1066
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1049..1064
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1085 AA;  121645 MW;  84C00A4E6B85DFB9 CRC64;
     MSYAELQVTT HFSFLRGASS AQELFETAKA LGIQAIGVVD RNSLAGIVRA LEASRATDIR
     LVVGCRLDLT DGMSLLVYPT DRAAYSRLTR LITLGKSRGG KNNCILHWDD VIAYSRGMIG
     VLVPDLPDAT CAAQLRRIAE AFGDRAYVSL CLRRRQNDQM RLHEISNLAA RFKVKTVVTN
     DVLFHEPGRR QLQDIVTCIR HNTTIDDVGF ERERHADRYL KPPEEMERLF SRYSEALART
     LEIVRRCKFS LEELTYQYPE EAIVPGKDAQ ASLEHYVWEC APDRYPEGLP QDVLKTVRHE
     LDLIRTMKYA PYFLTVFSIV RFARSQGILC QGRGSAANSA VCYILGITSI DPSTNDLLFE
     RFVSQERDEP PDIDVDFEHE RREEVIQWIY KTYTKDKAAL CATVTRYRAK GAIRDVGKAL
     GLPEDVIKAL SSGMWSWSEE VPDRNIRELN LNPDDRRLAL TLKLAQQLMG APRHLGQHPG
     GFVLTHDRLD DLVPIEPATM KDRQIIEWDK DDVEALKFMK VDVLALGMLT CMAKAFDLIR
     EHKGQQLDLS NIEQEDAATY AMIRKADTLG TFQIESRAQM AMLPRLKPRT FYDLVVQVAI
     VRPGPIQGDM VHPYLRRREG KEPVEYPTPE LEAVLGKTLG VPLFQESAMR VAMVCAGFTG
     GEADQLRKSM ATFKFTGGVS RFKDKLVSGM VKNGYSPEFA EKTFSQLEGF GSYGFPESHA
     ASFALIAYAS NYIKCHYPDV FCAALLNSQP MGFYAPAQIV GDAIKHGVEV RPVCVNRSRW
     DCTLERIEGS DRHAVRLGFR QVKGLAVADA ARIVAARMNN PFASVDDMWR RSSVPTEALV
     QLAEADAFLP SLKLERRDAL WAIKALRDEP LPLFAAAAER EATAIAEQQE PEVALRQMTD
     GHNVIEDYSH TGLTLRQHPV AFLRRDLSAR NIIPCAEAMN ARDGRWVYTA GLVLVRQKPG
     SAKGVMFITI EDETGPANIV VWPSLFEKRR SVVLGSSMMA INGRIQREGE VVHLVAQQLF
     DLSADLVGLA DRDTGFRLPA GRGDEFAHGG GGPDSRDRQK PVVPRDMFTP DLHIDTLKIK
     SRNFQ