ID   DNE22_RHIME             Reviewed;        1087 AA.
AC   Q92ZJ6;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Error-prone DNA polymerase 2 {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2-2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=RA0489;
GN   ORFNames=SMa0892;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE006469; AAK65147.1; -; Genomic_DNA.
DR   PIR; A95323; A95323.
DR   RefSeq; NP_435735.1; NC_003037.1.
DR   RefSeq; WP_010967470.1; NC_003037.1.
DR   AlphaFoldDB; Q92ZJ6; -.
DR   SMR; Q92ZJ6; -.
DR   EnsemblBacteria; AAK65147; AAK65147; SMa0892.
DR   GeneID; 61599288; -.
DR   KEGG; sme:SMa0892; -.
DR   PATRIC; fig|266834.11.peg.501; -.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; RGGKANC; -.
DR   Proteomes; UP000001976; Plasmid pSymA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0009432; P:SOS response; EXP:CollecTF.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1087
FT                   /note="Error-prone DNA polymerase 2"
FT                   /id="PRO_0000103396"
FT   REGION          1033..1064
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1087 AA;  121796 MW;  65E008BB06E2F98B CRC64;
     MRYAELQVTT HFSFLRAASS AEELFATARL MGIEALGVVD RNSLAGIVRA LEASRATGLR
     LVVGCRLDLQ DGMSILVYPT DRAAYSRLTR LLTLGKGRGG KANCIIHFDD VALYAEGLIG
     ILVPDLADEV CAVQLRKIAE VFGDRAYVSL CLRRRPNDQL QLHELTNLAV KHRVKTIVTN
     DVLFHEHGRR QLQDVVTCIR TGMTIDDVGF ERERHADRYL KPPEEMARLF PAYPEALART
     MEIVERCRFS LEELVYQYPE EALILGMTAQ QSLQHYTWEG VRARYPEGLP THVEKTIRHE
     LALIETMKYA PYFLTVFSIV RYARSQGILC QGRGSAANSA VCYVLGITSI DPETNDLLFE
     RFVSQERDEP PDIDVDFEHE RREEVIQWIY KTYGHDKAAL CSTVTRYRAK GAIRDVGKAL
     DLPEDLIRTL SSGIWSWSET VGERQVRELG LNPDDRRLTL TLRLAQQLMG APRNLSQHPG
     GFVLTHDRLD DLVPIEPATM ADRQVIEWDK DDIEALKFLK VDVLALGMLT CMAKAFALIS
     EHKHEDIDLA TIPQEDPATY AMIRKADTLG TFQIESRAQM SMLPRMKPRT FYDLVIQVAI
     VRPGPIQGDM VHPYLRRREG KEKVEYPTPE LEAVLHKTLG VPLFQESAMR VAMVCAGFTG
     GEADQLRKSM ATFKFTGGVS RFKDKLVNGM IRNGYTKEFA EKTFSQLEGF GSYGFPESHA
     ASFALIAYAS NYIKCYFPDV FCAALLNSQP MGFYAPAQIV RDAREHGVEV RPICINRSRW
     DCMLEPIDGS GGHAVRLGMR LVRGLATADA ARIVAARADE PFTSVDDMWR RSGVPVASLV
     ELAEADAFLP SLSLERRDAL WAIKALRDEP LPLFTAAADR EARAIAEQEE PEVELRQMTD
     GQNVVEDYSH TGLTLREHPL RFMRDDLAKR RIVTCAQAMT AHDGQWLMAA GLVLVRQRPG
     SAKGVMFITI EDETGIANIV VWPKLFERSR RVVLGASMMA INGRIQREGE VVHLVAQQLF
     DLSADLSSLA ERDGAFRPPT GRGDEFAHGS PGSADSRGKA PPGVRARDIL VPDLHIDTLK
     IKSRNFQ