ID   DNJ1_CRYNH              Reviewed;         522 AA.
AC   J9VKM5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1 {ECO:0000303|PubMed:34566931};
DE   Flags: Precursor;
GN   Name=DNJ1 {ECO:0000303|PubMed:34566931}; ORFNames=CNAG_01347;
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=34566931; DOI=10.3389/fmicb.2021.727039;
RA   Horianopoulos L.C., Lee C.W.J., Hu G., Caza M., Kronstad J.W.;
RT   "Dnj1 promotes virulence in Cryptococcus neoformans by maintaining robust
RT   endoplasmic reticulum homeostasis under temperature stress.";
RL   Front. Microbiol. 12:727039-727039(2021).
CC   -!- FUNCTION: Endoplasmic reticulum co-chaperone crucial for survival and
CC       virulence factor production at elevated temperatures representative of
CC       febrile patients during infection (PubMed:34566931). Contributes to
CC       virulence in a mouse model of cryptococcosis (PubMed:34566931). With
CC       chaperone CNE1, coordinately maintains ER homeostasis and contributes
CC       to maintenance of cell wall architecture (PubMed:34566931).
CC       {ECO:0000269|PubMed:34566931}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000269|PubMed:34566931}.
CC   -!- INDUCTION: Protein amounts are increased by ER stress elicited by
CC       tunicamycin. {ECO:0000269|PubMed:34566931}.
CC   -!- DOMAIN: The TPR repeats mediate interaction with unfolded polypeptides
CC       and the J-domain is essential to stimulate the ATPase activity of the
CC       chaperone and to increase its substrate affinity during the folding
CC       cycle. {ECO:0000250|UniProtKB:A0A0D1E2P6}.
CC   -!- DISRUPTION PHENOTYPE: Leads to hypersensitivity to endoplasmic
CC       reticulum (ER) stress caused by the N-glycosylation inhibitor
CC       tunicamycin (PubMed:34566931). Markedly impairs the elaboration of
CC       virulence factors such as the polysaccharide capsule and extracellular
CC       urease activity, when induced at 37 degrees Celsius corresponsing to
CC       the human body temperature (PubMed:34566931). Impairs also induction of
CC       the cytokines IL-6, IL-10, and MCP-1 in the lungs of mice and
CC       attenuates virulence in an intranasal murine model of cryptococcosis
CC       (PubMed:34566931). Leads to abnormal cell morphology of enlarged cells
CC       with collapsed cell walls and increased chitin within the cell walls,
CC       as well as strong growth defects at both 30 and 37 degrees Celsius when
CC       both DNJ1 and CNE1 are deleted (PubMed:34566931).
CC       {ECO:0000269|PubMed:34566931}.
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DR   EMBL; CP003824; AFR94798.1; -; Genomic_DNA.
DR   RefSeq; XP_012049486.1; XM_012194096.1.
DR   EnsemblFungi; AFR94798; AFR94798; CNAG_01347.
DR   GeneID; 23885071; -.
DR   VEuPathDB; FungiDB:CNAG_01347; -.
DR   HOGENOM; CLU_015935_0_1_1; -.
DR   Proteomes; UP000010091; Chromosome 5.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR001440; TPR_1.
DR   InterPro; IPR019734; TPR_repeat.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00515; TPR_1; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00028; TPR; 4.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS50005; TPR; 2.
PE   2: Evidence at transcript level;
KW   Chaperone; Endoplasmic reticulum; Repeat; Signal; TPR repeat; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..522
FT                   /note="Tetratricopeptide repeat and J domain-containing co-
FT                   chaperone DNJ1"
FT                   /id="PRO_5003827926"
FT   REPEAT          29..62
FT                   /note="TPR 1"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   REPEAT          63..96
FT                   /note="TPR 2"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   REPEAT          97..130
FT                   /note="TPR 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT   REPEAT          142..175
FT                   /note="TPR 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          210..243
FT                   /note="TPR 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          256..289
FT                   /note="TPR 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          356..389
FT                   /note="TPR 7"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   DOMAIN          410..471
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          465..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   522 AA;  58281 MW;  2BE3A3D83EA5D4B0 CRC64;
     MKGFLLVALP VLFLSLSTQV FGEPSIPSAA QIVQNANRLL AEGSYSAAAR AYGEAIELDP
     TGYANYYKRA TAYLSMGRHN AALDDFEQIL RINPGFVQAH YQRAKILAKE GDFAKAQYEL
     KAYVRTKSDS EAEELSHLLT VGEAAEKSAL QAFEKGKWQV CVEHSTKALE VGPNSEKLRR
     LRVNCATELG DINMVYGDLS RLASLDPSTT YLPLQLSNIA YFIRASSQAA AHIKQCLHFD
     PDSKPCKAVH KTIRSLEKDA ARVRNFIESG TYRQAIKILD GDDGLLVRFE KALDDATKPK
     DGLPPYLAPQ FHPKKNSQMR LDLYALACKA SVMANDFGEK GAHWCEETMS MNEENVDSWI
     SRGERLLRVE KWEEAMRAVE KAFELSGRSQ DILPRVQKAQ RLLKQSKQKD YYKVLGVPRD
     ADERAIKKAF RKAAKLAHPD VGGSEEKMAA LNEAYEVLSN TELRQRYDNG DDPNDPTGGQ
     QHNPFAHHGG GMPFQFFQQG GGFQGFHQGF PGGGQKMHFQ WN