ID   DNJ1_FUSO4              Reviewed;         521 AA.
AC   A0A0D2XVZ5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1 {ECO:0000303|PubMed:26487566};
DE   Flags: Precursor;
GN   Name=DNJ1 {ECO:0000303|PubMed:26487566}; ORFNames=FOXG_08159;
OS   Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935
OS   / NRRL 34936) (Fusarium vascular wilt of tomato).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium oxysporum species complex.
OX   NCBI_TaxID=426428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26487566; DOI=10.1111/nph.13703;
RA   Lo Presti L., Lopez Diaz C., Turra D., Di Pietro A., Hampel M., Heimel K.,
RA   Kahmann R.;
RT   "A conserved co-chaperone is required for virulence in fungal plant
RT   pathogens.";
RL   New Phytol. 209:1135-1148(2016).
CC   -!- FUNCTION: Endoplasmic reticulum co-chaperone required for the of
CC       virulence factors such as PG1, the major endopolygalacturonase produced
CC       during the infection of tomato plants. {ECO:0000269|PubMed:26487566}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000250|UniProtKB:A0A0D1E2P6}.
CC   -!- DOMAIN: The TPR repeats mediate interaction with unfolded polypeptides
CC       and the J-domain is essential to stimulate the ATPase activity of the
CC       chaperone and to increase its substrate affinity during the folding
CC       cycle. {ECO:0000250|UniProtKB:A0A0D1E2P6}.
CC   -!- DISRUPTION PHENOTYPE: Shows increased sensitivity to the N-
CC       glycosylation inhibitor tunicamycin and leads to a significant
CC       attenuation of the virulence on tomato plants (PubMed:26487566).
CC       Accumulates significantly less fungal biomass in infected plants and
CC       leads to significant decrease in secretion of PG1, the major
CC       endopolygalacturonase produced during the infection of tomato plants
CC       (PubMed:26487566). {ECO:0000269|PubMed:26487566}.
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DR   EMBL; DS231703; KNB06105.1; -; Genomic_DNA.
DR   RefSeq; XP_018244150.1; XM_018386940.1.
DR   STRING; 426428.A0A0D2XVZ5; -.
DR   EnsemblFungi; FOXG_08159T0; FOXG_08159P0; FOXG_08159.
DR   EnsemblFungi; KNB06105; KNB06105; FOXG_08159.
DR   GeneID; 28949807; -.
DR   KEGG; fox:FOXG_08159; -.
DR   VEuPathDB; FungiDB:FOXG_08159; -.
DR   OMA; PFAHFQH; -.
DR   PHI-base; PHI:5236; -.
DR   Proteomes; UP000009097; Chromosome 4.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF13181; TPR_8; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00028; TPR; 6.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS50005; TPR; 2.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Reference proteome; Repeat; Signal; TPR repeat;
KW   Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..521
FT                   /note="Tetratricopeptide repeat and J domain-containing co-
FT                   chaperone DNJ1"
FT                   /id="PRO_5010613517"
FT   REPEAT          33..66
FT                   /note="TPR 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT   REPEAT          67..100
FT                   /note="TPR 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT   REPEAT          102..134
FT                   /note="TPR 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          176..209
FT                   /note="TPR 4"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   REPEAT          211..244
FT                   /note="TPR 5"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   REPEAT          315..348
FT                   /note="TPR 6"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   REPEAT          349..382
FT                   /note="TPR 7"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00339"
FT   DOMAIN          404..473
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          464..521
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   521 AA;  56992 MW;  7ED234683F997807 CRC64;
     MHLNLAGLAV AATAFLATAS ALSPQDIPAD LPVSNLLTKA QTHLSRGETN EALVYYDAAI
     ARDPTNYLSL FKRATAYLSL GRTSQATEDF NKVLSLKPGF EGAHLQLARL RAKAGDWDAA
     KAQYGLAGKA PKSAEFVELE EAKLAAHLAD MASKGGKWEE CVSHAGTAIV VASRSPHLRE
     LRAHCRFELG DVELALSDLQ HVLHMKPGDT SPHIVISATS FYALGDLENG IGQVKKCLQS
     DPDSKICKKL HKQEKKVEKA YKKIQGQLSR GQPTTAGRAL VGTADDSGLV PDVRKQVEEL
     KKNKSIPKTA RIQLLENLIE MTCQAYTESS HKEAAKYCDE SLQLNPDSFW GLLHKGKAQL
     KSELYDAAIA TLEKAAEIRP DQKEKVNPIL NKAHIALKRS KTKDYYKVLG VENDADERQI
     KSAYRKQSKI FHPDKAAKQG IPKEEAEKKM ASINEAYEVL SDPELRARFD RGDDPNSQER
     PNPFQGQGNP FGGGHPFMFQ QGGGGGGPNI KFQFGGQPFG F