ID   DNJ36_ARATH             Reviewed;         482 AA.
AC   Q9LYW9;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 149.
DE   RecName: Full=DnaJ protein P58IPK homolog;
DE            Short=AtP58IPK;
DE   AltName: Full=Chaperone protein dnaJ 36;
DE            Short=AtDjA36;
DE            Short=AtJ36;
DE   Flags: Precursor;
GN   Name=P58IPK; Synonyms=A36; OrderedLocusNames=At5g03160;
GN   ORFNames=F15A17.90, MOK16.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11599562; DOI=10.1379/1466-1268(2001)006<0209:tjdpoa>2.0.co;2;
RA   Miernyk J.A.;
RT   "The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and
RT   diverse family of chaperones.";
RL   Cell Stress Chaperones 6:209-218(2001).
RN   [6]
RP   FUNCTION, INTERACTION WITH TOBAMOVIRUS TOBACCO ETCH VIRUS REPLICASES, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=12737801; DOI=10.1016/s1534-5807(03)00125-4;
RA   Bilgin D.D., Liu Y., Schiff M., Dinesh-Kumar S.P.;
RT   "P58(IPK), a plant ortholog of double-stranded RNA-dependent protein kinase
RT   PKR inhibitor, functions in viral pathogenesis.";
RL   Dev. Cell 4:651-661(2003).
RN   [7]
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY TUNICAMYCIN, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=18718935; DOI=10.1093/pcp/pcn119;
RA   Yamamoto M., Maruyama D., Endo T., Nishikawa S.;
RT   "Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulum
RT   that are conserved from yeast to animals and plants.";
RL   Plant Cell Physiol. 49:1547-1562(2008).
CC   -!- FUNCTION: Plays an important positive role in viral symptom development
CC       and is required for viral multiplication and pathogenesis.
CC       {ECO:0000269|PubMed:12737801}.
CC   -!- SUBUNIT: Interacts with the helicase domain of the tobamovirus (TMV)
CC       and the tobacco etch virus (TEV) replicases.
CC       {ECO:0000269|PubMed:12737801}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000305|PubMed:18718935}.
CC   -!- TISSUE SPECIFICITY: Expressed in flower buds and flowers.
CC       {ECO:0000269|PubMed:18718935}.
CC   -!- INDUCTION: By tunicamycin. {ECO:0000269|PubMed:18718935}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions, but the mutant plants die rapidly after challenges with the
CC       turnip mosaic virus (TuMV) and turnip vein clearing virus (TVCV). The
CC       double mutant erdj3b and p58ipk is male gametophytic lethal.
CC       {ECO:0000269|PubMed:12737801, ECO:0000269|PubMed:18718935}.
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DR   EMBL; AB005240; BAB08376.1; -; Genomic_DNA.
DR   EMBL; AL163002; CAB86083.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90562.1; -; Genomic_DNA.
DR   EMBL; AY085329; AAM62560.1; -; mRNA.
DR   PIR; T48337; T48337.
DR   RefSeq; NP_195936.1; NM_120394.4.
DR   AlphaFoldDB; Q9LYW9; -.
DR   SMR; Q9LYW9; -.
DR   STRING; 3702.AT5G03160.1; -.
DR   PaxDb; Q9LYW9; -.
DR   PRIDE; Q9LYW9; -.
DR   ProteomicsDB; 222086; -.
DR   EnsemblPlants; AT5G03160.1; AT5G03160.1; AT5G03160.
DR   GeneID; 831917; -.
DR   Gramene; AT5G03160.1; AT5G03160.1; AT5G03160.
DR   KEGG; ath:AT5G03160; -.
DR   Araport; AT5G03160; -.
DR   TAIR; locus:2143553; AT5G03160.
DR   eggNOG; KOG0550; Eukaryota.
DR   HOGENOM; CLU_015935_3_1_1; -.
DR   InParanoid; Q9LYW9; -.
DR   OMA; PFAHFQH; -.
DR   OrthoDB; 1106865at2759; -.
DR   PhylomeDB; Q9LYW9; -.
DR   PRO; PR:Q9LYW9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LYW9; baseline and differential.
DR   Genevisible; Q9LYW9; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:TAIR.
DR   GO; GO:0044794; P:positive regulation by host of viral process; IDA:UniProtKB.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR044620; P58IPK-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR013105; TPR_2.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR45188; PTHR45188; 1.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF07719; TPR_2; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00028; TPR; 5.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS50005; TPR; 5.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Host-virus interaction; Reference proteome; Repeat;
KW   Signal; TPR repeat.
FT   SIGNAL          1..42
FT                   /evidence="ECO:0000255"
FT   CHAIN           43..482
FT                   /note="DnaJ protein P58IPK homolog"
FT                   /id="PRO_0000430366"
FT   REPEAT          50..83
FT                   /note="TPR 1"
FT   REPEAT          85..117
FT                   /note="TPR 2"
FT   REPEAT          130..164
FT                   /note="TPR 3"
FT   REPEAT          166..198
FT                   /note="TPR 4"
FT   REPEAT          199..232
FT                   /note="TPR 5"
FT   REPEAT          245..278
FT                   /note="TPR 6"
FT   REPEAT          283..316
FT                   /note="TPR 7"
FT   REPEAT          318..350
FT                   /note="TPR 8"
FT   DOMAIN          370..436
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ   SEQUENCE   482 AA;  53690 MW;  695045261C692C5C CRC64;
     MDLFRVWSGM DFLAWRGMAY TLLLLNFVFA CQLLLLQPLV SALDGQSVDA AELFERASQS
     IKVKRYSDAL DDLNAAIEAD PALSEAYFKR ASVLRHFCRY EDSENSYQKY LEFKSGDSNA
     EKELSQLHQA KSALETASTL YESKDIAKAL EFVDKVVLVF SPACSKAKLL KVKLLMVSKD
     YSGAISETGY ILKEDENNLE ALLLRGRAYY YLADHDIAQR HYQKGLRLDP EHSELKKAYF
     GLKKLLKKTK SAEDNANKGK LRVSAEEYKE AIALDPEHTA NNVHLYLGLC KVSVRLGRGK
     DGLNSCNEAL NIDAELIEAL HQRGEAKLLL EDWEGAVEDL KQAAQNSQDM EIHESLGKAE
     KALKMSKRKD WYKILGISRT ASISEIKKAY KKLALQWHPD KNVGNREEAE NKFREIAAAY
     EILGDDDKRA RFDRGEDLED MGGGGGGGYN PFHGGGGGGQ QYTFHFEGGF PGGGGGFGGF
     GF