DNJ50_ARATH
ID DNJ50_ARATH Reviewed; 300 AA.
AC Q8GUN6; Q8RXY7; Q9SYB3;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Chaperone protein dnaJ 50;
DE Short=AtDjC50;
DE Short=AtJ50;
DE Flags: Precursor;
GN Name=C50; OrderedLocusNames=At1g61770; ORFNames=T13M11.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11599562; DOI=10.1379/1466-1268(2001)006<0209:tjdpoa>2.0.co;2;
RA Miernyk J.A.;
RT "The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and
RT diverse family of chaperones.";
RL Cell Stress Chaperones 6:209-218(2001).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND LACK OF INDUCTION
RP BY TUNICAMYCIN.
RX PubMed=18718935; DOI=10.1093/pcp/pcn119;
RA Yamamoto M., Maruyama D., Endo T., Nishikawa S.;
RT "Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulum
RT that are conserved from yeast to animals and plants.";
RL Plant Cell Physiol. 49:1547-1562(2008).
CC -!- FUNCTION: May play a role in protein folding in the endoplasmic
CC reticulum. {ECO:0000305|PubMed:18718935}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:18718935}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:18718935}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flower buds and flowers.
CC {ECO:0000269|PubMed:18718935}.
CC -!- INDUCTION: Not induced by tunicamycin.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21421.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005882; AAD21421.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33884.1; -; Genomic_DNA.
DR EMBL; AY080607; AAL86291.1; -; mRNA.
DR EMBL; BT002337; AAN86170.1; -; mRNA.
DR PIR; E96643; E96643.
DR RefSeq; NP_176370.2; NM_104859.3.
DR AlphaFoldDB; Q8GUN6; -.
DR SMR; Q8GUN6; -.
DR STRING; 3702.AT1G61770.1; -.
DR PaxDb; Q8GUN6; -.
DR PRIDE; Q8GUN6; -.
DR ProteomicsDB; 222089; -.
DR EnsemblPlants; AT1G61770.1; AT1G61770.1; AT1G61770.
DR GeneID; 842474; -.
DR Gramene; AT1G61770.1; AT1G61770.1; AT1G61770.
DR KEGG; ath:AT1G61770; -.
DR Araport; AT1G61770; -.
DR TAIR; locus:2195723; AT1G61770.
DR eggNOG; KOG0722; Eukaryota.
DR HOGENOM; CLU_055735_1_0_1; -.
DR InParanoid; Q8GUN6; -.
DR OMA; IGKAYRQ; -.
DR OrthoDB; 1332758at2759; -.
DR PhylomeDB; Q8GUN6; -.
DR PRO; PR:Q8GUN6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GUN6; baseline and differential.
DR Genevisible; Q8GUN6; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR044632; DNAJC25-like.
DR InterPro; IPR036869; J_dom_sf.
DR PANTHER; PTHR44176; PTHR44176; 1.
DR Pfam; PF00226; DnaJ; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..300
FT /note="Chaperone protein dnaJ 50"
FT /evidence="ECO:0000255"
FT /id="PRO_0000430367"
FT TOPO_DOM 28..122
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 123..143
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..206
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 207..227
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..300
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT DOMAIN 34..98
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 300 AA; 34952 MW; FFC7866CA7E2D263 CRC64;
MAPPVTERWC LALILLFLSL FVQSSTAIYC GAEDCYALLG VAQDANASDI KRSYYKLSLQ
HHPDKNPDPE SRKLFVKIAT AYEILKDNTT RAQYDYAIEH PEEVFYNTAQ YYRAKYGHKS
DPRAVLVGLL VVLSAFQYLN NVARYNEAIA TVKRTPAYKN KLKALELERT GGVSNKKKGS
KQIDQKLQEE LSNELDLQIK GAEKPSVWEL LGVRFILLPY TIIKLLVWYS SWVWRYKVKK
APYSWEDASY LTRRSLSVPA DAWANLDEYR KEDLVQKRLW EKQNLENYFA EMRKESKRRR
