DNJA4_MOUSE
ID DNJA4_MOUSE Reviewed; 397 AA.
AC Q9JMC3; B2RW09; Q543S9; Q9CTD6; Q9CUD4;
DT 11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=DnaJ homolog subfamily A member 4;
DE AltName: Full=MmDjA4;
DE Flags: Precursor;
GN Name=Dnaja4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1;
RX PubMed=10978524; DOI=10.1016/s0167-4781(00)00136-6;
RA Hata M., Ohtsuka K.;
RT "Murine cDNA encoding a novel type I HSP40/DNAJ homolog, mmDjA4.";
RL Biochim. Biophys. Acta 1493:208-210(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RC TISSUE=Brain, Corpora quadrigemina, Embryo, Spinal ganglion, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis and heart.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB30367.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB032401; BAA92775.1; -; mRNA.
DR EMBL; AK003903; BAB23067.1; -; mRNA.
DR EMBL; AK016666; BAB30367.2; ALT_INIT; mRNA.
DR EMBL; AK046476; BAC32747.1; -; mRNA.
DR EMBL; AK076175; BAC36232.1; -; mRNA.
DR EMBL; AK141909; BAE24880.1; -; mRNA.
DR EMBL; BC147484; AAI47485.1; -; mRNA.
DR EMBL; BC147486; AAI47487.1; -; mRNA.
DR CCDS; CCDS23193.1; -.
DR RefSeq; NP_067397.1; NM_021422.4.
DR AlphaFoldDB; Q9JMC3; -.
DR SMR; Q9JMC3; -.
DR BioGRID; 208406; 4.
DR IntAct; Q9JMC3; 1.
DR STRING; 10090.ENSMUSP00000070413; -.
DR iPTMnet; Q9JMC3; -.
DR PhosphoSitePlus; Q9JMC3; -.
DR REPRODUCTION-2DPAGE; Q9JMC3; -.
DR EPD; Q9JMC3; -.
DR MaxQB; Q9JMC3; -.
DR PaxDb; Q9JMC3; -.
DR PeptideAtlas; Q9JMC3; -.
DR PRIDE; Q9JMC3; -.
DR ProteomicsDB; 277348; -.
DR Antibodypedia; 27578; 144 antibodies from 25 providers.
DR DNASU; 58233; -.
DR Ensembl; ENSMUST00000070070; ENSMUSP00000070413; ENSMUSG00000032285.
DR Ensembl; ENSMUST00000120452; ENSMUSP00000112520; ENSMUSG00000032285.
DR GeneID; 58233; -.
DR KEGG; mmu:58233; -.
DR UCSC; uc009prk.2; mouse.
DR CTD; 55466; -.
DR MGI; MGI:1927638; Dnaja4.
DR VEuPathDB; HostDB:ENSMUSG00000032285; -.
DR eggNOG; KOG0712; Eukaryota.
DR GeneTree; ENSGT00940000155707; -.
DR HOGENOM; CLU_017633_10_0_1; -.
DR InParanoid; Q9JMC3; -.
DR OMA; NALCTKC; -.
DR PhylomeDB; Q9JMC3; -.
DR TreeFam; TF105141; -.
DR Reactome; R-MMU-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR BioGRID-ORCS; 58233; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Dnaja4; mouse.
DR PRO; PR:Q9JMC3; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9JMC3; protein.
DR Bgee; ENSMUSG00000032285; Expressed in seminiferous tubule of testis and 215 other tissues.
DR ExpressionAtlas; Q9JMC3; baseline and differential.
DR Genevisible; Q9JMC3; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0051087; F:chaperone binding; ISO:MGI.
DR GO; GO:0030544; F:Hsp70 protein binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051082; F:unfolded protein binding; ISO:MGI.
DR GO; GO:0010596; P:negative regulation of endothelial cell migration; ISO:MGI.
DR GO; GO:0090084; P:negative regulation of inclusion body assembly; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0042026; P:protein refolding; ISO:MGI.
DR GO; GO:0009408; P:response to heat; IEA:InterPro.
DR CDD; cd06257; DnaJ; 1.
DR CDD; cd10719; DnaJ_zf; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR HAMAP; MF_01152; DnaJ; 1.
DR InterPro; IPR012724; DnaJ.
DR InterPro; IPR002939; DnaJ_C.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR044713; DNJA1/2-like.
DR InterPro; IPR008971; HSP40/DnaJ_pept-bd.
DR InterPro; IPR001305; HSP_DnaJ_Cys-rich_dom.
DR InterPro; IPR036410; HSP_DnaJ_Cys-rich_dom_sf.
DR InterPro; IPR036869; J_dom_sf.
DR PANTHER; PTHR43888; PTHR43888; 1.
DR Pfam; PF00226; DnaJ; 1.
DR Pfam; PF01556; DnaJ_C; 1.
DR Pfam; PF00684; DnaJ_CXXCXGXG; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR SUPFAM; SSF49493; SSF49493; 2.
DR SUPFAM; SSF57938; SSF57938; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
DR PROSITE; PS51188; ZF_CR; 1.
PE 1: Evidence at protein level;
KW Chaperone; Lipoprotein; Membrane; Metal-binding; Methylation;
KW Phosphoprotein; Prenylation; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..394
FT /note="DnaJ homolog subfamily A member 4"
FT /id="PRO_0000071015"
FT PROPEP 395..397
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000396761"
FT DOMAIN 4..70
FT /note="J"
FT REPEAT 135..142
FT /note="CXXCXGXG motif"
FT REPEAT 151..158
FT /note="CXXCXGXG motif"
FT REPEAT 178..185
FT /note="CXXCXGXG motif"
FT REPEAT 194..201
FT /note="CXXCXGXG motif"
FT ZN_FING 122..206
FT /note="CR-type"
FT REGION 366..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..390
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 135
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 151
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 154
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 178
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 181
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 194
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW22"
FT MOD_RES 394
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 394
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 397 AA; 44902 MW; EF47E79FADDD9055 CRC64;
MVKETQYYDI LGVKPSASPE EIKKAYRKLA LKYHPDKNPD EGEKFKLISQ AYEVLSDPKK
RDIYDQGGEQ AIKEGGSGSP SFSSPMDIFD MFFGGGGRMT RERRGKNVVH QLSVTLEDLY
NGITKKLALQ KNVICEKCEG IGGKKGSVEK CPLCKGRGMQ VHIQQIGPGM VQQIQTVCIE
CKGQGERINP KDRCENCSGA KVTREKKIIE VHVEKGMKDG QKILFHGEGD QEPELDPGDV
IIVLDQKDHS VFQRRGQDLI MKMKIQLSEA LCGFKKTIKT LDDRVLVISS KSGEVIKHGD
LKCIRNEGMP IYKAPLEKGV MIIQFLVVFP EKQWLSQEKL PQLEALLPPR QKVRITDDMD
QVELKEFNPN EQSWRQHREA YEEDDEEPRA GVQCQTA
