ID   DNJB1_BOVIN             Reviewed;         340 AA.
AC   Q3MI00;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=DnaJ homolog subfamily B member 1;
GN   Name=DNAJB1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Interacts with HSP70 and can stimulate its ATPase activity.
CC       Stimulates the association between HSC70 and HIP. Negatively regulates
CC       heat shock-induced HSF1 transcriptional activity during the attenuation
CC       and recovery phase period of the heat shock response. Stimulates ATP
CC       hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B
CC       (in vitro). {ECO:0000250|UniProtKB:P25685}.
CC   -!- SUBUNIT: Interacts with DNAJC3. Interacts with HSF1 (via
CC       transactivation domain); this interaction results in the inhibition of
CC       heat shock- and HSF1-induced transcriptional activity during the
CC       attenuation and recovery phase period of the heat shock response.
CC       {ECO:0000250|UniProtKB:P25685}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P25685}. Nucleus
CC       {ECO:0000250|UniProtKB:P25685}. Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:P25685}. Note=Translocates rapidly from the
CC       cytoplasm to the nucleus, and especially to the nucleoli, upon heat
CC       shock. {ECO:0000250|UniProtKB:P25685}.
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DR   EMBL; BC104503; AAI04504.1; -; mRNA.
DR   RefSeq; NP_001028935.1; NM_001033763.1.
DR   AlphaFoldDB; Q3MI00; -.
DR   SMR; Q3MI00; -.
DR   STRING; 9913.ENSBTAP00000036132; -.
DR   PaxDb; Q3MI00; -.
DR   PRIDE; Q3MI00; -.
DR   Ensembl; ENSBTAT00000036270; ENSBTAP00000036132; ENSBTAG00000016874.
DR   GeneID; 538426; -.
DR   KEGG; bta:538426; -.
DR   CTD; 3337; -.
DR   VEuPathDB; HostDB:ENSBTAG00000016874; -.
DR   eggNOG; KOG0714; Eukaryota.
DR   GeneTree; ENSGT00940000160312; -.
DR   HOGENOM; CLU_017633_0_0_1; -.
DR   InParanoid; Q3MI00; -.
DR   OMA; KNNDLHQ; -.
DR   OrthoDB; 1393097at2759; -.
DR   TreeFam; TF105141; -.
DR   Reactome; R-BTA-3371453; Regulation of HSF1-mediated heat shock response.
DR   Reactome; R-BTA-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR   Reactome; R-BTA-5687128; MAPK6/MAPK4 signaling.
DR   Proteomes; UP000009136; Chromosome 7.
DR   Bgee; ENSBTAG00000016874; Expressed in bone marrow and 104 other tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0001671; F:ATPase activator activity; ISS:UniProtKB.
DR   GO; GO:0051117; F:ATPase binding; IEA:Ensembl.
DR   GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR   GO; GO:0030544; F:Hsp70 protein binding; IBA:GO_Central.
DR   GO; GO:0044183; F:protein folding chaperone; IEA:Ensembl.
DR   GO; GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR   GO; GO:0090084; P:negative regulation of inclusion body assembly; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0097201; P:negative regulation of transcription from RNA polymerase II promoter in response to stress; ISS:UniProtKB.
DR   GO; GO:0032781; P:positive regulation of ATP-dependent activity; IEA:Ensembl.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR002939; DnaJ_C.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR008971; HSP40/DnaJ_pept-bd.
DR   InterPro; IPR036869; J_dom_sf.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF01556; DnaJ_C; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF49493; SSF49493; 2.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   2: Evidence at transcript level;
KW   Chaperone; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW   Stress response.
FT   CHAIN           1..340
FT                   /note="DnaJ homolog subfamily B member 1"
FT                   /id="PRO_0000245486"
FT   DOMAIN          2..70
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          68..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         307
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P25685"
SQ   SEQUENCE   340 AA;  38217 MW;  F9E96B460F517DE6 CRC64;
     MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK
     REIFDRYGEE GLKGSGPSGG SSGGTNGTSF SYTFHGDPHA MFAEFFGGRN PFDNFFGQRN
     GEEGMDIDDP FSGFPMGMGG FTNMNFGRSR PAQEPTRKKQ DPPVTHDLRV SLEEIYSGCT
     KKMKISHKRL NPDGKSIRNE DKILTIEVKR GWKEGTKITF PKEGDQTSNN IPADIVFVLK
     DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE
     GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI