ID   DNJB3_HUMAN             Reviewed;         145 AA.
AC   Q8WWF6;
DT   13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=DnaJ homolog subfamily B member 3;
GN   Name=DNAJB3; Synonyms=HCG3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Hepatoma;
RA   Xueyuan D., Weifeng C.;
RT   "Cloning and identification of genes which are differentially expressed in
RT   hepatocellular carcinoma.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=18184612; DOI=10.1016/j.ygcen.2007.11.014;
RA   Meccariello R., Berruti G., Chianese R., De Santis R., Di Cunto F.,
RA   Scarpa D., Cobellis G., Zucchetti I., Pierantoni R., Altruda F., Fasano S.;
RT   "Structure of msj-1 gene in mice and humans: a possible role in the
RT   regulation of male reproduction.";
RL   Gen. Comp. Endocrinol. 156:91-103(2008).
RN   [5]
RP   STRUCTURE BY NMR OF 1-69.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the DNAj domain of the human protein hcg3, a
RT   hypothetical protein tmp_locus_21.";
RL   Submitted (FEB-2009) to the PDB data bank.
CC   -!- FUNCTION: May operate as a co-chaperone of the male germ cell- and
CC       haploid stage-specific Hsp70 proteins. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in sperm (at protein level).
CC       {ECO:0000269|PubMed:18184612}.
CC   -!- CAUTION: Due to a premature stop codon, the human protein is truncated
CC       and lacks the DNAJB3 canonical C-terminal domain. However, expression
CC       studies suggest the existence of the mRNA and protein in human.
CC       {ECO:0000305}.
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DR   EMBL; AY083665; AAM08934.1; -; mRNA.
DR   EMBL; AC114812; AAY24024.1; -; Genomic_DNA.
DR   EMBL; BC017590; AAH17590.1; -; mRNA.
DR   EMBL; BC024013; AAH24013.1; -; mRNA.
DR   RefSeq; NP_001001394.1; NM_001001394.3.
DR   PDB; 2EJ7; NMR; -; A=1-69.
DR   PDBsum; 2EJ7; -.
DR   AlphaFoldDB; Q8WWF6; -.
DR   SMR; Q8WWF6; -.
DR   BioGRID; 135928; 52.
DR   IntAct; Q8WWF6; 7.
DR   iPTMnet; Q8WWF6; -.
DR   PhosphoSitePlus; Q8WWF6; -.
DR   BioMuta; HGNC:32397; -.
DR   DMDM; 74730976; -.
DR   jPOST; Q8WWF6; -.
DR   MassIVE; Q8WWF6; -.
DR   MaxQB; Q8WWF6; -.
DR   PeptideAtlas; Q8WWF6; -.
DR   PRIDE; Q8WWF6; -.
DR   ProteomicsDB; 74882; -.
DR   DNASU; 414061; -.
DR   GeneID; 414061; -.
DR   CTD; 414061; -.
DR   DisGeNET; 414061; -.
DR   GeneCards; DNAJB3; -.
DR   HGNC; HGNC:32397; DNAJB3.
DR   neXtProt; NX_Q8WWF6; -.
DR   PharmGKB; PA162383828; -.
DR   InParanoid; Q8WWF6; -.
DR   OrthoDB; 1472647at2759; -.
DR   PhylomeDB; Q8WWF6; -.
DR   PathwayCommons; Q8WWF6; -.
DR   SignaLink; Q8WWF6; -.
DR   BioGRID-ORCS; 414061; 5 hits in 198 CRISPR screens.
DR   EvolutionaryTrace; Q8WWF6; -.
DR   GenomeRNAi; 414061; -.
DR   Pharos; Q8WWF6; Tdark.
DR   PRO; PR:Q8WWF6; -.
DR   Proteomes; UP000005640; Unplaced.
DR   RNAct; Q8WWF6; protein.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR   GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0061077; P:chaperone-mediated protein folding; IBA:GO_Central.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR026901; DNAJB3.
DR   InterPro; IPR036869; J_dom_sf.
DR   PANTHER; PTHR43948:SF1; PTHR43948:SF1; 1.
DR   Pfam; PF00226; DnaJ; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Reference proteome.
FT   CHAIN           1..145
FT                   /note="DnaJ homolog subfamily B member 3"
FT                   /id="PRO_0000386472"
FT   DOMAIN          1..69
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   VARIANT         85
FT                   /note="D -> E (in dbSNP:rs34622615)"
FT                   /id="VAR_061142"
FT   VARIANT         98
FT                   /note="D -> E (in dbSNP:rs34150486)"
FT                   /id="VAR_061143"
FT   HELIX           4..7
FT                   /evidence="ECO:0007829|PDB:2EJ7"
FT   HELIX           16..27
FT                   /evidence="ECO:0007829|PDB:2EJ7"
FT   TURN            32..34
FT                   /evidence="ECO:0007829|PDB:2EJ7"
FT   HELIX           39..56
FT                   /evidence="ECO:0007829|PDB:2EJ7"
FT   HELIX           61..67
FT                   /evidence="ECO:0007829|PDB:2EJ7"
SQ   SEQUENCE   145 AA;  16559 MW;  065CD71109B6F0DE CRC64;
     MVDYYEVLDV PRQASSEAIK KAYRKLALKW HPDKNPENKE EAERRFKQVA EAYEVLSDAK
     KRDIYDRYGE AGAEGGCTGG RPFEDPFEYV FSFRDPADVF REFFGGQDPF SFDLLGNPLE
     NILGGSEELL GKQKQSVCTP FLCLQ