DNJB5_HUMAN
ID DNJB5_HUMAN Reviewed; 348 AA.
AC O75953; B3KN14; B4DSA6; J3KQM9; J3KR08; Q5T656; Q8TDR7; Q96EM4;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=DnaJ homolog subfamily B member 5;
DE AltName: Full=Heat shock protein Hsp40-2;
DE AltName: Full=Heat shock protein Hsp40-3;
DE AltName: Full=Heat shock protein cognate 40;
DE Short=Hsc40;
GN Name=DNAJB5; Synonyms=HSC40;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Colon;
RX PubMed=10570961; DOI=10.1016/s0378-1119(99)00333-9;
RA Chen M.-S., Roti J.R., Laszlo A.;
RT "Hsc40, a new member of the hsp40 family, exhibits similar expression
RT profile to that of hsc70 in mammalian cells.";
RL Gene 238:333-341(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Fu Q., Yu L., Yue P., Zhou Y., Jiang J.X., Zhao S.Y.;
RT "Cloning and expression of a new human cDNA homology to human heat-shock
RT protein 40 mRNA.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC O75953; O00555: CACNA1A; NbExp=2; IntAct=EBI-5655937, EBI-766279;
CC O75953; Q8WZ42: TTN; NbExp=4; IntAct=EBI-5655937, EBI-681210;
CC O75953; Q8AZK7: EBNA-LP; Xeno; NbExp=3; IntAct=EBI-5655937, EBI-1185167;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O75953-3; Sequence=Displayed;
CC Name=2;
CC IsoId=O75953-4; Sequence=VSP_046223;
CC Name=3;
CC IsoId=O75953-5; Sequence=VSP_047250;
CC -!- INDUCTION: Expressed under normal conditions, its expression can
CC further be increased after various stress treatments.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH12115.1; Type=Miscellaneous discrepancy; Note=Unlikely isoform. Aberrant splice sites.; Evidence={ECO:0000305};
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DR EMBL; AF088982; AAC35860.1; -; mRNA.
DR EMBL; AF087870; AAM10498.1; -; mRNA.
DR EMBL; AK023253; BAG51176.1; -; mRNA.
DR EMBL; AK299647; BAG61568.1; -; mRNA.
DR EMBL; AL355377; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471071; EAW58407.1; -; Genomic_DNA.
DR EMBL; BC012115; AAH12115.1; ALT_SEQ; mRNA.
DR CCDS; CCDS35007.1; -. [O75953-3]
DR CCDS; CCDS47959.1; -. [O75953-4]
DR RefSeq; NP_001128476.2; NM_001135004.2.
DR RefSeq; NP_001128477.1; NM_001135005.2. [O75953-4]
DR RefSeq; NP_036398.3; NM_012266.5. [O75953-3]
DR RefSeq; XP_005251485.1; XM_005251428.3. [O75953-3]
DR RefSeq; XP_006716814.1; XM_006716751.2.
DR RefSeq; XP_011516144.1; XM_011517842.2.
DR AlphaFoldDB; O75953; -.
DR SMR; O75953; -.
DR BioGRID; 117350; 115.
DR IntAct; O75953; 38.
DR MINT; O75953; -.
DR STRING; 9606.ENSP00000404079; -.
DR iPTMnet; O75953; -.
DR PhosphoSitePlus; O75953; -.
DR BioMuta; DNAJB5; -.
DR EPD; O75953; -.
DR jPOST; O75953; -.
DR MassIVE; O75953; -.
DR MaxQB; O75953; -.
DR PaxDb; O75953; -.
DR PeptideAtlas; O75953; -.
DR PRIDE; O75953; -.
DR ProteomicsDB; 50319; -. [O75953-3]
DR Antibodypedia; 11397; 191 antibodies from 28 providers.
DR DNASU; 25822; -.
DR Ensembl; ENST00000312316.9; ENSP00000312517.5; ENSG00000137094.16. [O75953-3]
DR Ensembl; ENST00000454002.6; ENSP00000413684.2; ENSG00000137094.16. [O75953-4]
DR Ensembl; ENST00000545841.5; ENSP00000441999.1; ENSG00000137094.16. [O75953-3]
DR Ensembl; ENST00000682809.1; ENSP00000507741.1; ENSG00000137094.16. [O75953-4]
DR Ensembl; ENST00000684748.1; ENSP00000506753.1; ENSG00000137094.16. [O75953-3]
DR GeneID; 25822; -.
DR KEGG; hsa:25822; -.
DR MANE-Select; ENST00000682809.1; ENSP00000507741.1; NM_001349723.3; NP_001336652.1. [O75953-4]
DR UCSC; uc003zvs.5; human. [O75953-3]
DR CTD; 25822; -.
DR DisGeNET; 25822; -.
DR GeneCards; DNAJB5; -.
DR HGNC; HGNC:14887; DNAJB5.
DR HPA; ENSG00000137094; Tissue enhanced (skeletal muscle, tongue).
DR MIM; 611328; gene.
DR neXtProt; NX_O75953; -.
DR OpenTargets; ENSG00000137094; -.
DR PharmGKB; PA27417; -.
DR VEuPathDB; HostDB:ENSG00000137094; -.
DR eggNOG; KOG0714; Eukaryota.
DR GeneTree; ENSGT00940000156090; -.
DR HOGENOM; CLU_017633_0_0_1; -.
DR InParanoid; O75953; -.
DR OMA; DVHWTLA; -.
DR OrthoDB; 1393097at2759; -.
DR PhylomeDB; O75953; -.
DR TreeFam; TF105141; -.
DR PathwayCommons; O75953; -.
DR SignaLink; O75953; -.
DR BioGRID-ORCS; 25822; 10 hits in 1069 CRISPR screens.
DR ChiTaRS; DNAJB5; human.
DR GenomeRNAi; 25822; -.
DR Pharos; O75953; Tbio.
DR PRO; PR:O75953; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; O75953; protein.
DR Bgee; ENSG00000137094; Expressed in hindlimb stylopod muscle and 165 other tissues.
DR ExpressionAtlas; O75953; baseline and differential.
DR Genevisible; O75953; HS.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0051087; F:chaperone binding; IPI:UniProtKB.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0006986; P:response to unfolded protein; IEP:UniProtKB.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR002939; DnaJ_C.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR008971; HSP40/DnaJ_pept-bd.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR Pfam; PF01556; DnaJ_C; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR SUPFAM; SSF49493; SSF49493; 2.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chaperone; Reference proteome.
FT CHAIN 1..348
FT /note="DnaJ homolog subfamily B member 5"
FT /id="PRO_0000071023"
FT DOMAIN 4..68
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT VAR_SEQ 1
FT /note="M -> MFKRTVLSCPPPAAPPLQARGAFRSFPHSWGEDFLASLMFKIQLEPL
FT KLRAWTLNGFVKFRNKETSAGPVAVM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_046223"
FT VAR_SEQ 1
FT /note="M -> MGGAEAEPWGRAPGPAIGGRRAGDSCPGWRRRSRSRGRGQRLSHGPR
FT RRPQLLTAAPPLQARGAFRSFPHSWGEDFLASLMFKIQLEPLKLRAWTLNGFVKFRNKE
FT TSAGPVAVM (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_047250"
FT CONFLICT 190..196
FT /note="MKITRRR -> IEDHKAS (in Ref. 2; AAM10498)"
FT /evidence="ECO:0000305"
FT CONFLICT 234..235
FT /note="TP -> HL (in Ref. 2; AAM10498)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 348 AA; 39133 MW; DC9FE45DE4FD8CFC CRC64;
MGKDYYKILG IPSGANEDEI KKAYRKMALK YHPDKNKEPN AEEKFKEIAE AYDVLSDPKK
RGLYDQYGEE GLKTGGGTSG GSSGSFHYTF HGDPHATFAS FFGGSNPFDI FFASSRSTRP
FSGFDPDDMD VDEDEDPFGA FGRFGFNGLS RGPRRAPEPL YPRRKVQDPP VVHELRVSLE
EIYHGSTKRM KITRRRLNPD GRTVRTEDKI LHIVIKRGWK EGTKITFPKE GDATPDNIPA
DIVFVLKDKP HAHFRRDGTN VLYSALISLK EALCGCTVNI PTIDGRVIPL PCNDVIKPGT
VKRLRGEGLP FPKVPTQRGD LIVEFKVRFP DRLTPQTRQI LKQHLPCS
