DNJC8_MOUSE
ID DNJC8_MOUSE Reviewed; 253 AA.
AC Q6NZB0; Q6PG72; Q8C2M6;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=DnaJ homolog subfamily C member 8;
GN Name=Dnajc8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic germ cell, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Suppresses polyglutamine (polyQ) aggregation of ATXN3 in
CC neuronal cells. {ECO:0000250|UniProtKB:O75937}.
CC -!- SUBUNIT: Interacts with SRPK1. Interacts with HSP70 (HSPA1A or HSPA1B).
CC {ECO:0000250|UniProtKB:O75937}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75937}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC40283.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK088325; BAC40283.1; ALT_FRAME; mRNA.
DR EMBL; BC057191; AAH57191.1; -; mRNA.
DR EMBL; BC066222; AAH66222.2; -; mRNA.
DR CCDS; CCDS51319.1; -.
DR RefSeq; NP_765988.2; NM_172400.3.
DR AlphaFoldDB; Q6NZB0; -.
DR SMR; Q6NZB0; -.
DR BioGRID; 212944; 13.
DR STRING; 10090.ENSMUSP00000092242; -.
DR iPTMnet; Q6NZB0; -.
DR PhosphoSitePlus; Q6NZB0; -.
DR EPD; Q6NZB0; -.
DR MaxQB; Q6NZB0; -.
DR PaxDb; Q6NZB0; -.
DR PRIDE; Q6NZB0; -.
DR ProteomicsDB; 279460; -.
DR Antibodypedia; 30906; 92 antibodies from 20 providers.
DR DNASU; 68598; -.
DR Ensembl; ENSMUST00000094657; ENSMUSP00000092242; ENSMUSG00000054405.
DR GeneID; 68598; -.
DR KEGG; mmu:68598; -.
DR UCSC; uc012dmi.1; mouse.
DR CTD; 22826; -.
DR MGI; MGI:1915848; Dnajc8.
DR VEuPathDB; HostDB:ENSMUSG00000054405; -.
DR eggNOG; KOG1150; Eukaryota.
DR GeneTree; ENSGT00390000012569; -.
DR InParanoid; Q6NZB0; -.
DR OMA; NPDNQER; -.
DR OrthoDB; 1434217at2759; -.
DR PhylomeDB; Q6NZB0; -.
DR TreeFam; TF105167; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 68598; 22 hits in 75 CRISPR screens.
DR ChiTaRS; Dnajc8; mouse.
DR PRO; PR:Q6NZB0; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q6NZB0; protein.
DR Bgee; ENSMUSG00000054405; Expressed in otic placode and 260 other tissues.
DR ExpressionAtlas; Q6NZB0; baseline and differential.
DR Genevisible; Q6NZB0; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0045171; C:intercellular bridge; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030544; F:Hsp70 protein binding; ISO:MGI.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR042858; DNAJC8.
DR InterPro; IPR036869; J_dom_sf.
DR PANTHER; PTHR15606; PTHR15606; 1.
DR Pfam; PF00226; DnaJ; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chaperone; Nucleus; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT CHAIN 2..253
FT /note="DnaJ homolog subfamily C member 8"
FT /id="PRO_0000071061"
FT DOMAIN 57..124
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT REGION 181..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..253
FT /note="Essential for polyglutamine aggregation suppression"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT MOTIF 189..192
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT MOTIF 203..206
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT COMPBIAS 181..221
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT MOD_RES 146
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT MOD_RES 222
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75937"
FT CONFLICT 151
FT /note="E -> G (in Ref. 1; BAC40283)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 253 AA; 29813 MW; 295D0C3F0B2A0F49 CRC64;
MAASGESGAS GGGGSTEEAF MTFYSEVKQI EKRDSVLTSK NQIERLTRPG SSYFNLNPFE
VLQIDPEVTD EEIKKRFRQL SILVHPDKNQ DDADRAQKAF EAVDKAYKLL LDQEQKKRAL
DVIQAGKEYV EHTVKERKKQ LKKEGKPTNV EEDDPELFKQ AVYKQTMKLF AELEIKRKER
EAKEMHERKR QREEEIEAQE KAKREREWQK NFEESRDGRV DSWRNFQANT KGKKEKKNRT
FLRPPKVKME QRE
