DNLI_OPITP
ID DNLI_OPITP Reviewed; 1017 AA.
AC B1ZZL9;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Probable DNA ligase;
DE EC=6.5.1.1 {ECO:0000255|PROSITE-ProRule:PRU10135};
DE AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN Name=lig; OrderedLocusNames=Oter_3148;
OS Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1).
OC Bacteria; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus.
OX NCBI_TaxID=452637;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11246 / JCM 15787 / PB90-1;
RX PubMed=21398538; DOI=10.1128/jb.00228-11;
RA van Passel M.W., Kant R., Palva A., Copeland A., Lucas S., Lapidus A.,
RA Glavina del Rio T., Pitluck S., Goltsman E., Clum A., Sun H., Schmutz J.,
RA Larimer F.W., Land M.L., Hauser L., Kyrpides N., Mikhailova N.,
RA Richardson P.P., Janssen P.H., de Vos W.M., Smidt H.;
RT "Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an
RT abundant inhabitant of rice paddy soil ecosystems.";
RL J. Bacteriol. 193:2367-2368(2011).
CC -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA during DNA
CC replication, DNA recombination and DNA repair. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10135};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SIMILARITY: In the C-terminal section; belongs to the ATP-dependent DNA
CC ligase family. {ECO:0000305}.
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DR EMBL; CP001032; ACB76428.1; -; Genomic_DNA.
DR RefSeq; WP_012375957.1; NC_010571.1.
DR AlphaFoldDB; B1ZZL9; -.
DR SMR; B1ZZL9; -.
DR STRING; 452637.Oter_3148; -.
DR PRIDE; B1ZZL9; -.
DR EnsemblBacteria; ACB76428; ACB76428; Oter_3148.
DR KEGG; ote:Oter_3148; -.
DR eggNOG; COG1236; Bacteria.
DR eggNOG; COG1793; Bacteria.
DR HOGENOM; CLU_296629_0_0_0; -.
DR OMA; KYDGIRC; -.
DR OrthoDB; 1073940at2; -.
DR Proteomes; UP000007013; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3260.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.60.15.10; -; 1.
DR InterPro; IPR000977; DNA_ligase_ATP-dep.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR InterPro; IPR036599; DNA_ligase_N_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF04675; DNA_ligase_A_N; 1.
DR SUPFAM; SSF117018; SSF117018; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
DR TIGRFAMs; TIGR00574; dnl1; 1.
DR PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; DNA damage; DNA recombination;
KW DNA repair; DNA replication; Ligase; Magnesium; Metal-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..1017
FT /note="Probable DNA ligase"
FT /id="PRO_0000365242"
FT REGION 1..363
FT /note="Unknown"
FT REGION 326..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..1017
FT /note="DNA ligase"
FT ACT_SITE 669
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10135"
FT BINDING 667
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 674
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 689
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 717
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 860
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 866
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1017 AA; 109840 MW; F2D76752C20C30B9 CRC64;
MPWDVKFSHG LYLPQLGWWL DAHFPQKRSF VSHAHSDHTA THDEILCSAG TAALMRARLD
AKRIEHVLPF GQTEQLTADC GVTLHPAGHI FGSAQCLLEH GEHGSLLYTG DFKLRRGRSA
EPCATPHAEL VIMETTFGRP HYVFPPTAQV LNDIATFCHE TIADDGVPVL FGYSLGKSQE
LLCSLEESQL PVMLHPQTHK LTRVYEQFGI AFPPYREFDL ATVRGHVVIC PPQSRESAFV
RRIPGHRTAM ITGWAMDPGA IYRYQCDAAF PLSDHADYQD LLRFVEAVNP QRVLTLHGFA
TEFAQTLRER GLEAWAIGED NQLELGIRSS PPQVRAGDAT PSSRSSGDAG VAAPACATPL
HAPDSFARFV AAADAVKATP KKLEKIAVLR DYLAALTPPD MGTAATFLTG RAFPQRDPRN
LTLGWSVIKR AVLEVAGVTE ADYRDAYHRF ADTGDAAGAV LAQRSLRPGD ATPSSRPEIL
GVAGVADPGP AASRPAATSA PTTCSLADMA LFFERAAAAR GPAAKLDLLR ERLALITPDE
ARYLIKIITG DLRIGLKEGL VEEALAAAAG QPLEAVREAA MLCGDIAAVA RAARTDTLAE
IQLTVFNPLQ FMLASPEPTA EAIVDRFAAQ RTAEAAAIVA GVGDPGPGSS SPATADAAPA
LARIWLEEKY DGIRCQLHKS GGRVELYSRD LNVITEQFPD LARAALALPH DFIGDGELLA
WRDGRALPFA ELQKRLGRKG GDDFFLGAEI PVSISFYDLL WLDGRSLLKE PLRERRALLE
RLLHGDATPS SRPDGDEGVA APALQPATLN PQLSTKFSLA PVTFAHTASD IEAAFLAARQ
RGNEGLMAKD PASGYTPGRR GLAWLKLKKA YATLDVVVVG VEYGHGKRRD VLSDYTFAIR
DEEHDNQLLT VGKAYSGLTD VEIAQLTQHF LEHTLEVHGR YRVVVPDTVI EVAFDTIQPS
SRHQSGFALR FPRIARIRTD KTPAEIDTLA TCRKLAAAAG SGGIAAVGAR PPPAASD
