ID   DNMK_BPT5               Reviewed;         250 AA.
AC   Q6QGP4; Q66M07;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Deoxynucleoside-5'-monophosphate kinase {ECO:0000312|EMBL:AAS77090.1};
DE            EC=2.7.4.13 {ECO:0000269|PubMed:14711503};
GN   Name=dnk {ECO:0000312|EMBL:AAS77090.1};
GN   ORFNames=T5.043 {ECO:0000312|EMBL:AAS77090.1},
GN   T5p042 {ECO:0000312|EMBL:AAU05195.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   SUBUNIT.
RX   PubMed=14711503; DOI=10.1016/j.pep.2003.07.006;
RA   Mikoulinskaia G.V., Zimin A.A., Feofanov S.A., Miroshnikov A.I.;
RT   "Identification, cloning, and expression of bacteriophage T5 dnk gene
RT   encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC
RT   2.7.4.13).";
RL   Protein Expr. Purif. 33:166-175(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX11976.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [5]
RP   FUNCTION.
RX   PubMed=5846986; DOI=10.1016/s0021-9258(18)97004-3;
RA   Orr C.W., Herriott S.T., Bessman M.J.;
RT   "The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic
RT   acid polymerase induced by bacteriophage T5.";
RL   J. Biol. Chem. 240:4652-4658(1965).
RN   [6]
RP   MUTAGENESIS OF SER-13; ASP-16; THR-17; ARG-130; LYS-131; GLN-134; GLY-137;
RP   THR-138; TRP-150; ASP-170; ARG-172 AND GLU-176.
RX   PubMed=25696930;
RA   Mikulinskaia G.V., Taran S.A., Skoblov I.U.S., Feofanov S.A.;
RT   "[Investigation of active center of deoxynucleoside monophosphate kinase of
RT   bacteriophage T5 by site-directed mutagenesis].";
RL   Bioorg. Khim. 39:680-692(2013).
CC   -!- FUNCTION: Allows the synthesis of deoxyribonucleoside triphosphates
CC       necessary for the rapid viral DNA replication (PubMed:14711503).
CC       Phosphorylates all four dNMPs (PubMed:5846986).
CC       {ECO:0000269|PubMed:14711503, ECO:0000269|PubMed:5846986}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-phosphate + ATP = a 2'-
CC         deoxyribonucleoside 5'-diphosphate + ADP; Xref=Rhea:RHEA:11216,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:65317, ChEBI:CHEBI:73316,
CC         ChEBI:CHEBI:456216; EC=2.7.4.13;
CC         Evidence={ECO:0000269|PubMed:14711503};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:14711503}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305|PubMed:15661140}.
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DR   EMBL; AY509815; AAS19390.1; -; Genomic_DNA.
DR   EMBL; AY543070; AAS77090.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX11976.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05195.1; -; Genomic_DNA.
DR   RefSeq; YP_006871.1; NC_005859.1.
DR   SMR; Q6QGP4; -.
DR   GeneID; 2777591; -.
DR   KEGG; vg:2777591; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0047507; F:(deoxy)nucleoside-phosphate kinase activity; IDA:UniProtKB.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0046939; P:nucleotide phosphorylation; IDA:CACAO.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   DNA replication; Early protein; Kinase; Reference proteome; Transferase;
KW   Viral DNA replication.
FT   CHAIN           1..250
FT                   /note="Deoxynucleoside-5'-monophosphate kinase"
FT                   /id="PRO_0000435563"
FT   MUTAGEN         13
FT                   /note="S->A: About 5-50% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         16
FT                   /note="D->N: About 5-50% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         17
FT                   /note="T->N: About 75-95% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         17
FT                   /note="T->S: About 5-50% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         130
FT                   /note="R->K: Complete loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         131
FT                   /note="K->E: Almost no loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         134
FT                   /note="Q->A: About 75-95% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         137
FT                   /note="G->A: Almost complete loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         138
FT                   /note="T->A: About 75-95% loss of enzyme activity depending
FT                   on the substrate."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         150
FT                   /note="W->A,F: No loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         170
FT                   /note="D->N: Complete loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         172
FT                   /note="R->I: Complete loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   MUTAGEN         176
FT                   /note="E->Q: Almost complete loss of enzyme activity."
FT                   /evidence="ECO:0000269|PubMed:25696930"
FT   CONFLICT        237
FT                   /note="V -> M (in Ref. 4; AAU05195)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   250 AA;  28701 MW;  E2FE0D8E7A86CFCA CRC64;
     MSVLVGLHGE AGSGKDTVAK LIIDWCNDTY PTCLSRRYSF AKPVYELASV ILGVTPEFLG
     ERRGKEIDQW FTVTQSQLER ARDVWFKYGI DKFEDFSYVW PIFEEKYLNP QQLISENKED
     GLYSLFISPR KMLQLVGTEL GRQLVHERIW LIILEQSIAK DDPDVAVITD VRFPNEGELL
     RETNHLDMDS LLVNVVPAEQ KFTIKSDHPS ESGIPAKYIT HELVNKFDGI NNLKLEVYNF
     CDLELEPLVG