ID   DNRE_BPT4               Reviewed;         392 AA.
AC   P04523; Q94N08; Q96224;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   02-JUN-2021, entry version 100.
DE   RecName: Full=DNA replication protein;
DE   AltName: Full=Gene product 69;
DE            Short=gp69;
GN   Name=69;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6098451; DOI=10.1002/j.1460-2075.1984.tb02221.x;
RA   McDonald P.M., Mosig G.;
RT   "Regulation of a new bacteriophage T4 gene, 69, that spans an origin of DNA
RT   replication.";
RL   EMBO J. 3:2863-2871(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-392.
RC   STRAIN=GT7;
RX   PubMed=1692800; DOI=10.1016/0378-1119(90)90053-t;
RA   Frazier M.W., Mosig G.;
RT   "The bacteriophage T4 gene mrh whose product inhibits late T4 gene
RT   expression in an Escherichia coli rpoH (sigma 32) mutant.";
RL   Gene 88:7-14(1990).
RN   [4]
RP   FUNCTION.
RX   PubMed=3023622; DOI=10.1016/0022-2836(86)90395-5;
RA   Mosig G., Macdonald P.;
RT   "A new membrane-associated DNA replication protein, the gene 69 product of
RT   bacteriophage T4, shares a patch of homology with the Escherichia coli dnaA
RT   protein.";
RL   J. Mol. Biol. 189:243-248(1986).
CC   -!- FUNCTION: May play a role in viral DNA replication. Found associated
CC       with a viral DNA origin of replication and with host membranes, may
CC       attach this origin to the bacterial envelope to initiate replication.
CC       {ECO:0000269|PubMed:3023622}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative promoter usage; Named isoforms=2;
CC       Name=Long; Synonyms=GP69;
CC         IsoId=P04523-1; Sequence=Displayed;
CC       Name=Short; Synonyms=GP69*;
CC         IsoId=P04523-2; Sequence=VSP_018677;
CC   -!- MISCELLANEOUS: [Isoform Long]: Expression of isoform Long is delayed
CC       since its middle promoter requires T4 coded proteins for activation.
CC   -!- MISCELLANEOUS: [Isoform Short]: Can be expressed immediately from an
CC       E.coli-like promoter. {ECO:0000305}.
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DR   EMBL; X01416; CAA25661.1; -; Genomic_DNA.
DR   EMBL; X01416; CAA25662.1; -; Genomic_DNA.
DR   EMBL; K03113; AAA32556.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42517.1; -; Genomic_DNA.
DR   EMBL; M30001; AAB07792.1; -; Genomic_DNA.
DR   PIR; A04299; Z9BPT4.
DR   PIR; T10133; T10133.
DR   RefSeq; NP_049645.1; NC_000866.4. [P04523-2]
DR   GeneID; 1258803; -.
DR   KEGG; vg:1258803; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR000305; GIY-YIG_endonuc.
DR   SMART; SM00465; GIYc; 1.
PE   4: Predicted;
KW   Alternative promoter usage; DNA replication; Reference proteome.
FT   CHAIN           1..392
FT                   /note="DNA replication protein"
FT                   /id="PRO_0000003324"
FT   REGION          322..341
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..168
FT                   /note="Missing (in isoform Short)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018677"
FT   CONFLICT        356
FT                   /note="S -> F (in Ref. 1; CAA25661/CAA25662/AAA32556)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   392 AA;  46179 MW;  740AE210731D0161 CRC64;
     MAHFNECAHL IEGVDKAQNE YWDILGDEKD PLQVMLDMQR FLQIRLANVR EYCYHPDKLE
     TAGDVVSWMR EQKDCIDDEF RELLTSLGEM SRGEKEASAV WKKWKARYIE AQEKRIDEMS
     PEDQLEIKFE LVDIFHFVLN MFVGLGMNAE EIFKLYYLKN KHILNVKIMD IKQKFYRTYI
     VKVRTPKGVF WYAGKHESFI VNPYNDKYPG SGKILWNIYR KYGFNYKIRW SKCHGSREKS
     YEVERELISA LKRKHPDTCI NISPGGQGGE GRKWTEQQRL EHKLRLNNPE TKTRMKNSQR
     IAQNRAERKA RQSEVMKKFY SNGGNKKISE GTSRAQRKAP HWHEPLKSEI HELWVSLGKP
     ATGPVVKALK GKYDVTSSAL KNLIYLFRKE DV