ID   DNRJ_STRPE              Reviewed;         370 AA.
AC   P25048;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Daunorubicin biosynthesis sensory transduction protein DnrJ;
GN   Name=dnrJ;
OS   Streptomyces peucetius.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1950;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972;
RX   PubMed=1729206; DOI=10.1128/jb.174.1.144-154.1992;
RA   Stutzman-Engwall K.J., Otten S.L., Hutchinson C.R.;
RT   "Regulation of secondary metabolism in Streptomyces spp. and overproduction
RT   of daunorubicin in Streptomyces peucetius.";
RL   J. Bacteriol. 174:144-154(1992).
CC   -!- FUNCTION: May form, with DnrI, a two-component regulatory system for
CC       daunorubicin biosynthesis genes. This protein probably acts as a sensor
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DegT/DnrJ/EryC1 family. {ECO:0000305}.
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DR   EMBL; M80237; AAA26737.1; -; Genomic_DNA.
DR   PIR; B43306; B43306.
DR   AlphaFoldDB; P25048; -.
DR   SMR; P25048; -.
DR   BioCyc; MetaCyc:MON-19960; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   CDD; cd00616; AHBA_syn; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000653; DegT/StrS_aminotransferase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR30244; PTHR30244; 1.
DR   Pfam; PF01041; DegT_DnrJ_EryC1; 1.
DR   PIRSF; PIRSF000390; PLP_StrS; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Cell membrane; DNA-binding; Membrane;
KW   Pyridoxal phosphate; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..370
FT                   /note="Daunorubicin biosynthesis sensory transduction
FT                   protein DnrJ"
FT                   /id="PRO_0000110011"
FT   DNA_BIND        160..179
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          53..158
FT                   /note="Hydrophobic"
FT   MOD_RES         186
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   370 AA;  40999 MW;  6B8674E5B0DD082B CRC64;
     MSTYVWQYLN EYREERADIL DAVETVFESG QLILGTSVRS FEEEFAAYHG LPYCTGVDNG
     TNALVLGLRA LGIGPGDEVV TVSNTAAPTV VAIDAVGATP VFVDVHEENY LMDTGRLRSV
     IGPRTRCLLP VHLYGQSVDM TPVLELAAEH DLKVLEDCAQ AHGARRHGRL VGTQGHAAAF
     SFYPTKVLGA YGDGGAVVTP DAEVDRRLRR LRYYGMGERY YVVDTPGHNS RLDEVQAEIL
     RRKLRRLDAY VEGRRAVARR YEEGLGDLDG LVLPTIAEGN DHVYYVYVVR HPERDRILEA
     LTAYDIHLNI SYPWPVHTMS GFAHLGYGPG DLPVTERLAG EIFSLPMYPS LRPDAQEKVI
     DAVREVVGSL