ID   DNRU_STRPE              Reviewed;         287 AA.
AC   Q9ZAU1;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Putative daunorubicin C-13 ketoreductase DnrU;
DE            EC=1.1.1.-;
GN   Name=dnrU;
OS   Streptomyces peucetius.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1950;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PUTATIVE FUNCTION.
RC   STRAIN=ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972;
RX   PubMed=9864344; DOI=10.1128/jb.181.1.305-318.1999;
RA   Lomovskaya N., Otten S.L., Doi-Katayama Y., Fonstein L., Liu X.-C.,
RA   Takatsu T., Inventi A., Filippi S., Torti F., Colombo A.L.,
RA   Hutchinson C.R.;
RT   "Doxorubicin overproduction in Streptomyces peucetius: cloning and
RT   characterization of the dnrU ketoreductase and dnrV genes and the doxA
RT   cytochrome P-450 hydroxylase gene.";
RL   J. Bacteriol. 181:305-318(1999).
CC   -!- FUNCTION: Could reduce the 13-carbonyl of daunorubicin to yield (13S)-
CC       13-dihydrodaunorubicin. Could also be able to reduce the 13-carbonyl of
CC       doxorubicin.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; U77891; AAD04717.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9ZAU1; -.
DR   SMR; Q9ZAU1; -.
DR   KEGG; ag:AAD04717; -.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IMP:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN           1..287
FT                   /note="Putative daunorubicin C-13 ketoreductase DnrU"
FT                   /id="PRO_0000425688"
FT   ACT_SITE        175
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         24..30
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  30632 MW;  4F9448EAE55F6191 CRC64;
     MTASTPHHGT PRGGLSGRTV LVTGATSGIG RAAALAVARQ GARVVLVGRD PERLRTVTNE
     VARTAGPAPD AFRADFAELR QVRDLGERLR DRYPRIDVMA SNAGGMFWSR TTTQDGFEAT
     IQVNHLAGFL LARLLRERLA GGRLILTSSD AYTQGRIDPD DLNGDRHRYS AGQAYGTSKQ
     ANIMTAAEAA RRWPDVLAVS YHPGEVRTRI GRGTVASSYF RFNPFLRSAA KGADTLVWLA
     SAPAEELTTG GYYSDRRLSP VSGPTADAGL AAKLWEAGAA AVGDTAH