ID   DNRU_STRS5              Reviewed;         287 AA.
AC   Q53877;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Putative daunorubicin C-13 ketoreductase DnrU;
DE            EC=1.1.1.-;
OS   Streptomyces sp. (strain C5).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=45212;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C5;
RX   PubMed=8655529; DOI=10.1128/jb.178.11.3384-3388.1996;
RA   Dickens M.L., Ye J., Strohl W.R.;
RT   "Cloning, sequencing, and analysis of aklaviketone reductase from
RT   Streptomyces sp. strain C5.";
RL   J. Bacteriol. 178:3384-3388(1996).
CC   -!- FUNCTION: Could reduce the 13-carbonyl of daunorubicin to produce
CC       (13S)-13-dihydrodaunorubicin. Could also be able to reduce the 13-
CC       carbonyl of doxorubicin (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; U43704; AAB08016.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q53877; -.
DR   SMR; Q53877; -.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; ISS:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN           1..287
FT                   /note="Putative daunorubicin C-13 ketoreductase DnrU"
FT                   /id="PRO_0000425689"
FT   ACT_SITE        175
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         24..30
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  30800 MW;  8FEB9D11BAFE1E78 CRC64;
     MTVPTPRHGT PHGGLPGRTV LITGATSGIG RAAALAIARQ GARVVLVGRD PERLRTVTNE
     VARTAGPAPD AFRADFAELR QVRELGERLR DRYPRIDVMA GNAGGMFWSR TTTQDGFEAT
     LQVNHLAGFL LARLLRERLA GGRLILTSSD AYTQGRIDPD DLNGDRHRYS AGQAYGTSKQ
     ANIMTATEAA RRWPDVLTVS YHPGEVRTRI GRGTVASTYF RFNPFLRSAA KGADTLVWLA
     AAPAEELTTG GYYSDRRLSP VSGPTADAGL AAKLWEASAA AVGDTAR