ID   DNRV_STRPE              Reviewed;         275 AA.
AC   Q9ZAU2;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Anthracycline biosynthesis protein DnrV;
GN   Name=dnrV;
OS   Streptomyces peucetius.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1950;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972;
RX   PubMed=9864344; DOI=10.1128/jb.181.1.305-318.1999;
RA   Lomovskaya N., Otten S.L., Doi-Katayama Y., Fonstein L., Liu X.-C.,
RA   Takatsu T., Inventi A., Filippi S., Torti F., Colombo A.L.,
RA   Hutchinson C.R.;
RT   "Doxorubicin overproduction in Streptomyces peucetius: cloning and
RT   characterization of the dnrU ketoreductase and dnrV genes and the doxA
RT   cytochrome P-450 hydroxylase gene.";
RL   J. Bacteriol. 181:305-318(1999).
CC   -!- FUNCTION: Involved in the biosynthesis of the anthracyclines
CC       carminomycin and daunorubicin (daunomycin) which are aromatic
CC       polyketide antibiotics that exhibit high cytotoxicity and are widely
CC       applied in the chemotherapy of a variety of cancers. In vivo, it acts
CC       jointly with DoxA in the conversion of 13-deoxycarminomycin and 13-
CC       deoxydaunorubicin to yield carminomycin and daunorubicin, respectively.
CC       In vitro, it also acts jointly with DoxA in the C-14 hydroxylation of
CC       daunorubicin to form doxorubicin, although this strain is not a
CC       doxorubicin producer. {ECO:0000269|PubMed:9864344}.
CC   -!- PATHWAY: Antibiotic biosynthesis; daunorubicin biosynthesis.
CC   -!- PATHWAY: Antibiotic biosynthesis; carminomycin biosynthesis.
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DR   EMBL; U77891; AAD04716.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9ZAU2; -.
DR   SMR; Q9ZAU2; -.
DR   UniPathway; UPA00054; -.
DR   UniPathway; UPA01040; -.
DR   GO; GO:1901771; P:daunorubicin biosynthetic process; IMP:UniProtKB.
DR   Gene3D; 3.10.180.10; -; 2.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR041581; Glyoxalase_6.
DR   InterPro; IPR037523; VOC.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   Pfam; PF18029; Glyoxalase_6; 1.
DR   SUPFAM; SSF54593; SSF54593; 2.
DR   PROSITE; PS51819; VOC; 2.
PE   4: Predicted;
KW   Antibiotic biosynthesis; Repeat.
FT   CHAIN           1..275
FT                   /note="Anthracycline biosynthesis protein DnrV"
FT                   /id="PRO_0000425686"
FT   DOMAIN          8..136
FT                   /note="VOC 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   DOMAIN          150..263
FT                   /note="VOC 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
SQ   SEQUENCE   275 AA;  28481 MW;  DEE13C0E714D4397 CRC64;
     MTRFAPGAPA WFDLGSPDVA ASADFYTGLF GWTATVVSDP GAGGYTTFSS DGKLVAAVAR
     HQIDTPYHRP YGPGNDQHGM PAIWTVYFAT DDADALTKRV ETAGGEVIMT PMDVLGLGRM
     AVFADPAGAA FAVWRKGVME GAEVTGVPGS VGWVELVTDG IGAARDFYPA TLGLAPADTG
     LKGVTDPVWH IGDTPVAGTQ ELGVTGAVRP HWAVLFAVHD CDATVRRAVE LGGSVENEPA
     DTPRGRRADL LDPHGAGFSV VELREGYPAA AGGAS