ID   DNS2B_RAT               Reviewed;         356 AA.
AC   Q9QZK9;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Deoxyribonuclease-2-beta;
DE            EC=3.1.22.1;
DE   AltName: Full=DNase II-like acid DNase;
DE   AltName: Full=DNase2-like acid DNase;
DE   AltName: Full=Deoxyribonuclease II beta;
DE            Short=DNase II beta;
DE   AltName: Full=Endonuclease DLAD;
DE   Flags: Precursor;
GN   Name=Dnase2b; Synonyms=Dlad;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=Wistar; TISSUE=Liver;
RX   PubMed=10558878; DOI=10.1006/bbrc.1999.1699;
RA   Tanuma S., Shiokawa D.;
RT   "Cloning of a cDNA encoding a rat DNase II-like acid DNase.";
RL   Biochem. Biophys. Res. Commun. 265:395-399(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 48-71 AND 211-219, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RA   Lubec G., Kang S.U., Lubec S.;
RL   Submitted (SEP-2007) to UniProtKB.
CC   -!- FUNCTION: Hydrolyzes DNA under acidic conditions. Does not require
CC       divalent cations for activity. Participates in the degradation of
CC       nuclear DNA during lens cell differentiation.
CC       {ECO:0000269|PubMed:10558878}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-
CC         phosphooligonucleotide end-products.; EC=3.1.22.1;
CC   -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Liver specific. {ECO:0000269|PubMed:10558878}.
CC   -!- MISCELLANEOUS: Inhibited by aurintricarboxylic acid and Zn(2+).
CC   -!- SIMILARITY: Belongs to the DNase II family. {ECO:0000305}.
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DR   EMBL; AF178974; AAF13596.1; -; mRNA.
DR   PIR; JC7131; JC7131.
DR   RefSeq; NP_067696.1; NM_021664.1.
DR   AlphaFoldDB; Q9QZK9; -.
DR   SMR; Q9QZK9; -.
DR   STRING; 10116.ENSRNOP00000021861; -.
DR   GlyGen; Q9QZK9; 4 sites.
DR   PaxDb; Q9QZK9; -.
DR   PRIDE; Q9QZK9; -.
DR   GeneID; 59296; -.
DR   KEGG; rno:59296; -.
DR   CTD; 58511; -.
DR   RGD; 70901; Dnase2b.
DR   eggNOG; KOG3825; Eukaryota.
DR   InParanoid; Q9QZK9; -.
DR   PhylomeDB; Q9QZK9; -.
DR   BRENDA; 3.1.22.1; 5301.
DR   PRO; PR:Q9QZK9; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005576; C:extracellular region; ISO:RGD.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004531; F:deoxyribonuclease II activity; IDA:RGD.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; ISO:RGD.
DR   GO; GO:0006309; P:apoptotic DNA fragmentation; IBA:GO_Central.
DR   InterPro; IPR004947; DNase_II.
DR   PANTHER; PTHR10858; PTHR10858; 1.
DR   Pfam; PF03265; DNase_II; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Glycoprotein; Hydrolase; Lysosome;
KW   Nuclease; Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..356
FT                   /note="Deoxyribonuclease-2-beta"
FT                   /id="PRO_0000007297"
FT   CARBOHYD        77
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        98
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        114
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        273
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   356 AA;  40472 MW;  5CBC259F42DD6741 CRC64;
     MTAQPLKAAL PLLFVALSGV LGTPVISCIN EDGKAVDWFA FYKLPRRTSR GGTGMGLDYL
     YLDSTMRTWS KSHHLINSSR SSLGRTLEQL YEAHNAKNDT AYLIYNDAVP ASVNYSGNYG
     HAKGLLVWNR VQGFWLIHSI PKFPPVPEKG YEYPSSGRQY AQSGLCITLK YSQYETIDSQ
     LLVFQPNIYS CFIPNIFRWE LIHMPQMCAK SSASKIPSRR LTVLQSAQGL NFLHFAKSTF
     YTDDIFAAWI AQKLKVHLLV ESWQRKNHEL PSNCSLPYHV YNIKAIRGPL QSDFPSHHDH
     SKWCVSTKDS QARWTCIGDL NRSPHQALRS GGFICSKNRY IYQSFDRLVS HYASCN