ID   DNS2_BURTA              Reviewed;         350 AA.
AC   Q2T8B0;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Putative deoxyribonuclease-2;
DE            EC=3.1.-.-;
DE   AltName: Full=Deoxyribonuclease II;
GN   OrderedLocusNames=BTH_II0389;
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS   E264).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
CC   -!- MISCELLANEOUS: Unlike their eukaryotic orthologs the putative
CC       Burkholderia deoxyribonuclease-2 are probably not secreted from the
CC       cell, as they do not have a signal sequence.
CC   -!- SIMILARITY: Belongs to the DNase II family. {ECO:0000305}.
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DR   EMBL; CP000085; ABC35537.1; -; Genomic_DNA.
DR   RefSeq; WP_009895252.1; NZ_CP008786.1.
DR   PDB; 5I3E; X-ray; 1.65 A; A/B=1-350.
DR   PDB; 5UNB; X-ray; 1.75 A; A/B=1-350.
DR   PDBsum; 5I3E; -.
DR   PDBsum; 5UNB; -.
DR   AlphaFoldDB; Q2T8B0; -.
DR   SMR; Q2T8B0; -.
DR   PRIDE; Q2T8B0; -.
DR   EnsemblBacteria; ABC35537; ABC35537; BTH_II0389.
DR   KEGG; bte:BTH_II0389; -.
DR   HOGENOM; CLU_053867_0_0_4; -.
DR   OMA; ILYNDQK; -.
DR   OrthoDB; 449055at2; -.
DR   BRENDA; 3.1.22.1; 8156.
DR   Proteomes; UP000001930; Chromosome II.
DR   GO; GO:0004531; F:deoxyribonuclease II activity; IEA:InterPro.
DR   InterPro; IPR004947; DNase_II.
DR   PANTHER; PTHR10858; PTHR10858; 1.
DR   Pfam; PF03265; DNase_II; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..350
FT                   /note="Putative deoxyribonuclease-2"
FT                   /id="PRO_0000230670"
FT   STRAND          14..20
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          24..27
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          30..33
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          38..41
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   TURN            43..45
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           61..70
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          77..82
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           88..90
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          103..108
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   TURN            109..112
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          113..118
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          127..129
FT                   /evidence="ECO:0007829|PDB:5UNB"
FT   STRAND          136..147
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           148..160
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          166..170
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           179..183
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          192..201
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          207..213
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           221..224
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           226..230
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          260..263
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           265..267
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          272..274
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           275..277
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          282..287
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          290..295
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          307..313
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           315..322
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   STRAND          325..327
FT                   /evidence="ECO:0007829|PDB:5I3E"
FT   HELIX           335..344
FT                   /evidence="ECO:0007829|PDB:5I3E"
SQ   SEQUENCE   350 AA;  38700 MW;  78D1E06635F135C6 CRC64;
     MAISPRDEQN RSVDLWFAYK VPKLTKDADS DSASGYEYVY YDRQVGAVQK SPNLMNDPKG
     ALFYTLDSVF GDPGDTTGWI LYNDEMPADA NRSNNATLGH TKGVIAFDIA SSSALWLLHS
     WPKYASPSVP GVPTPLYGQT FLCLSLDLAT AGKLAAQMAL HQQPQVYLPR TGGLDHTSPL
     YALTQPLNAS APGDSDSLDF KTRGGVPFKV IAKNRKWGKD FWNDLVGPTL KADMYVETWI
     RGKIPPVLDS DGVHKTYDIK FIDLRKLGAP WAWPETQDHA KWGITTTDNW VCVGDINRMV
     TQEKRGGGTI AFQDPKLWKA LCETDLIIPP PGKTDAQARA MIRKTHEPAE