ID   DNTAB_BURSR             Reviewed;         104 AA.
AC   Q45694;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=2,4-dinitrotoluene dioxygenase system, ferredoxin component {ECO:0000303|PubMed:8759857};
GN   Name=dntAb {ECO:0000303|PubMed:8759857};
OS   Burkholderia sp. (strain RASC).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=69003;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBSTRATE SPECIFICITY, AND
RP   SUBUNIT.
RC   STRAIN=DNT {ECO:0000312|EMBL:AAB09765.1};
RX   PubMed=8759857; DOI=10.1128/jb.178.16.4926-4934.1996;
RA   Suen W.C., Haigler B.E., Spain J.C.;
RT   "2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT:
RT   similarity to naphthalene dioxygenase.";
RL   J. Bacteriol. 178:4926-4934(1996).
CC   -!- FUNCTION: Component of the 2,4-dinitrotoluene dioxygenase (DNTDO)
CC       multicomponent enzyme system which catalyzes the incorporation of both
CC       atoms of molecular oxygen into 2,4-dinitrotoluene (DNT) to form 4-
CC       methyl-5-nitrocatechol (MNC) and nitrite. Functions as an intermediate
CC       electron transfer protein via a specific interaction with iron sulfur
CC       protein components (ISP)(DntAc and DntAd). Also able to convert
CC       naphthalene to cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene.
CC       {ECO:0000269|PubMed:8759857}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000250|UniProtKB:P0A185,
CC         ECO:0000255|PROSITE-ProRule:PRU00628};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:P0A185, ECO:0000255|PROSITE-ProRule:PRU00628};
CC   -!- SUBUNIT: The 2,4-dinitrotoluene dioxygenase (DNTDO) multicomponent
CC       enzyme system is composed of an electron transfer component and a
CC       dioxygenase component (iron sulfur protein (ISP)). The electron
CC       transfer component is composed of a ferredoxin reductase (DntAa) and a
CC       ferredoxin (DntAb), and the dioxygenase component is formed of a large
CC       alpha subunit (DntAc) and a small beta subunit (DntAd).
CC       {ECO:0000305|PubMed:8759857}.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       ferredoxin component family. {ECO:0000305}.
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DR   EMBL; U62430; AAB09765.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45694; -.
DR   SMR; Q45694; -.
DR   KEGG; ag:AAB09765; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IDA:UniProtKB.
DR   Gene3D; 2.102.10.10; -; 1.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   Pfam; PF00355; Rieske; 1.
DR   SUPFAM; SSF50022; SSF50022; 1.
DR   PROSITE; PS51296; RIESKE; 1.
PE   1: Evidence at protein level;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Transport.
FT   CHAIN           1..104
FT                   /note="2,4-dinitrotoluene dioxygenase system, ferredoxin
FT                   component"
FT                   /id="PRO_0000442191"
FT   DOMAIN          5..101
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250|UniProtKB:P0A185,
FT                   ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         47
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250|UniProtKB:P0A185,
FT                   ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         64
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250|UniProtKB:P0A185,
FT                   ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         67
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250|UniProtKB:P0A185,
FT                   ECO:0000255|PROSITE-ProRule:PRU00628"
SQ   SEQUENCE   104 AA;  11906 MW;  A57A464D17B739D7 CRC64;
     MSENWIDAAA RDEVPRGRRD RHQYRRQGDC LYEVAGEIYA TDNTCTHGAA RMSDGFLEGR
     EIECPLHQGR FDVCTGKALC TPLTQDIKTY PVKIENMRVM LKLD