ID   DNTAD_BURSR             Reviewed;         194 AA.
AC   Q45696;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=2,4-dinitrotoluene dioxygenase system, small oxygenase component {ECO:0000305};
DE   AltName: Full=2,4-dinitrotoluene dioxygenase ISP beta {ECO:0000305};
DE   AltName: Full=2,4-dinitrotoluene dioxygenase subunit beta {ECO:0000303|PubMed:8759857};
GN   Name=dntAd {ECO:0000303|PubMed:8759857};
OS   Burkholderia sp. (strain RASC).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=69003;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBSTRATE SPECIFICITY, AND
RP   SUBUNIT.
RC   STRAIN=DNT {ECO:0000312|EMBL:AAB09767.1};
RX   PubMed=8759857; DOI=10.1128/jb.178.16.4926-4934.1996;
RA   Suen W.C., Haigler B.E., Spain J.C.;
RT   "2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT:
RT   similarity to naphthalene dioxygenase.";
RL   J. Bacteriol. 178:4926-4934(1996).
CC   -!- FUNCTION: Component of the 2,4-dinitrotoluene dioxygenase (DNTDO)
CC       multicomponent enzyme system which catalyzes the incorporation of both
CC       atoms of molecular oxygen into 2,4-dinitrotoluene (DNT) to form 4-
CC       methyl-5-nitrocatechol (MNC) and nitrite. The beta subunit seems to
CC       have a structural role in the holoenzyme. Also able to convert
CC       naphthalene to cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene.
CC       {ECO:0000269|PubMed:8759857}.
CC   -!- SUBUNIT: The 2,4-dinitrotoluene dioxygenase (DNTDO) multicomponent
CC       enzyme system is composed of an electron transfer component and a
CC       dioxygenase component (iron sulfur protein (ISP)). The electron
CC       transfer component is composed of a ferredoxin reductase (DntAa) and a
CC       ferredoxin (DntAb), and the dioxygenase component is formed of a large
CC       alpha subunit (DntAc) and a small beta subunit (DntAd).
CC       {ECO:0000305|PubMed:8759857}.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       beta subunit family. {ECO:0000305}.
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DR   EMBL; U62430; AAB09767.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45696; -.
DR   SMR; Q45696; -.
DR   KEGG; ag:AAB09767; -.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   PANTHER; PTHR41534; PTHR41534; 1.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Oxidoreductase.
FT   CHAIN           1..194
FT                   /note="2,4-dinitrotoluene dioxygenase system, small
FT                   oxygenase component"
FT                   /id="PRO_0000442190"
SQ   SEQUENCE   194 AA;  23037 MW;  F08C488CC0AA61A5 CRC64;
     MMINTQEDKL VSAHDAEEFH RFFVGHDSDL QQEVTTLLTR EADLLDIQAY KAWLEHCVAP
     EIKYQVISRE LRSTSERRYQ LNDAVNIYNE NYQQLKVRVE HQMDPQNWYN SPKIRFTRFV
     TNVTAAKDKS APEMLHVRSN LILHRARRGN QVDVFYATRE DKWKRIEGGG IKLVERFVDY
     PERSPQTHNL MIFL