ID   DODA_AMAMU              Reviewed;         228 AA.
AC   P87064;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=DOPA 4,5-dioxygenase;
DE            EC=1.14.99.-;
GN   Name=DODA;
OS   Amanita muscaria (Fly agaric).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX   NCBI_TaxID=41956;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 90-103; 140-158;
RP   178-188 AND 206-215.
RC   TISSUE=Cap;
RX   PubMed=9341673; DOI=10.1007/s004380050539;
RA   Hinz U.G., Fivaz J., Girod P.-A., Zryd J.-P.;
RT   "The gene coding for the DOPA dioxygenase involved in betalain biosynthesis
RT   in Amanita muscaria and its regulation.";
RL   Mol. Gen. Genet. 256:1-6(1997).
RN   [2]
RP   CHARACTERIZATION.
RA   Mueller L.A., Hinz U.G., Uze M., Sautter C., Zryd J.-P.;
RT   "Biochemical complementation of the betalain biosynthetic pathway in
RT   Portulaca grandiflora by a fungal 3,4-dihydroxyphenylalanine dioxygenase.";
RL   Planta 203:260-263(1997).
RN   [3]
RP   CHARACTERIZATION.
RA   Mueller L.A., Hinz U.G., Zryd J.-P.;
RT   "The formation of betalamic acid and muscaflavin by recombinant DOPA-
RT   dioxygenase from amanita.";
RL   Phytochemistry 44:567-569(1997).
CC   -!- FUNCTION: Extradiol dioxygenase that opens up the cyclic ring of DOPA
CC       between carbons 4 and 5 thus producing an unstable seco-DOPA that
CC       rearranges non-enzymatically to betalamic acid. Can also catalyze the
CC       formation of muscaflavin (a pigment found in the hygrocybe mushrooms
CC       family and of some amanita species only) by a 2,3-extradiol cleavage of
CC       DOPA.
CC   -!- PATHWAY: Pigment biosynthesis; betalain biosynthesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Expressed at high level in coloured cap tissue and
CC       at least 10 times lower level in the stipe.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Pretty pigments - Issue 1 of
CC       September 2000;
CC       URL="https://web.expasy.org/spotlight/back_issues/001";
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DR   EMBL; Y12886; CAA73387.1; -; Genomic_DNA.
DR   AlphaFoldDB; P87064; -.
DR   SMR; P87064; -.
DR   KEGG; ag:CAA73387; -.
DR   UniPathway; UPA00278; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1240; -; 1.
DR   InterPro; IPR023389; DOPA-like_sf.
DR   InterPro; IPR014980; DOPA_dioxygen.
DR   PANTHER; PTHR36423; PTHR36423; 1.
DR   Pfam; PF08883; DOPA_dioxygen; 1.
DR   SUPFAM; SSF143410; SSF143410; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Dioxygenase; Direct protein sequencing; Oxidoreductase.
FT   CHAIN           1..228
FT                   /note="DOPA 4,5-dioxygenase"
FT                   /id="PRO_0000079972"
SQ   SEQUENCE   228 AA;  26176 MW;  C03CF0F055026E10 CRC64;
     MVPSFVVYSS WVNGRQRYIR QAFASILFYI IRDTTLSFPS HTTMSTKPET DLQTVLDSEI
     KEWHFHIYFH QNNAAEHQAA LELRDAVLRL RQDGAFVAVP LFRVNMDPMG PHPVGSYEIW
     VPSETFASVF SYLCMNRGRL SILVHPLTRE ELRDHEIRNA WIGPSFPLNL ANLPIKSDEI
     PLQYPSLKLG YSSTAHKMSL EERRKLGDDI EAVLRGEKEA ARAPHRDA