DOF21_ARATH
ID DOF21_ARATH Reviewed; 288 AA.
AC Q8LE43; Q8VYM9; Q9SK07;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Dof zinc finger protein DOF2.1 {ECO:0000303|PubMed:12475498};
DE Short=AtDOF2.1 {ECO:0000303|PubMed:12475498};
GN Name=DOF2.1 {ECO:0000303|PubMed:12475498};
GN OrderedLocusNames=At2g28510 {ECO:0000312|Araport:AT2G28510};
GN ORFNames=T17D12.7 {ECO:0000312|EMBL:AAD21486.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [6]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20563990; DOI=10.1387/ijdb.093006jg;
RA Gardiner J., Sherr I., Scarpella E.;
RT "Expression of DOF genes identifies early stages of vascular development in
RT Arabidopsis leaves.";
RL Int. J. Dev. Biol. 54:1389-1396(2010).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00071}.
CC -!- TISSUE SPECIFICITY: Accumulates in the stele.
CC {ECO:0000269|PubMed:20563990}.
CC -!- DEVELOPMENTAL STAGE: Expressed at preprocambial stages first in wide
CC domains, and later confined to sites of vein development.
CC {ECO:0000269|PubMed:20563990}.
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DR EMBL; AC006587; AAD21486.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08133.1; -; Genomic_DNA.
DR EMBL; AY070421; AAL49917.1; -; mRNA.
DR EMBL; BT001954; AAN71953.1; -; mRNA.
DR EMBL; AY085635; AAM62856.1; -; mRNA.
DR PIR; G84685; G84685.
DR RefSeq; NP_565673.1; NM_128411.3.
DR AlphaFoldDB; Q8LE43; -.
DR BioGRID; 2749; 1.
DR IntAct; Q8LE43; 1.
DR STRING; 3702.AT2G28510.1; -.
DR iPTMnet; Q8LE43; -.
DR PaxDb; Q8LE43; -.
DR PRIDE; Q8LE43; -.
DR ProteomicsDB; 222107; -.
DR DNASU; 817399; -.
DR EnsemblPlants; AT2G28510.1; AT2G28510.1; AT2G28510.
DR GeneID; 817399; -.
DR Gramene; AT2G28510.1; AT2G28510.1; AT2G28510.
DR KEGG; ath:AT2G28510; -.
DR Araport; AT2G28510; -.
DR TAIR; locus:2056588; AT2G28510.
DR eggNOG; ENOG502RB9Y; Eukaryota.
DR HOGENOM; CLU_036438_2_1_1; -.
DR InParanoid; Q8LE43; -.
DR OMA; GMSLEGN; -.
DR OrthoDB; 1111544at2759; -.
DR PhylomeDB; Q8LE43; -.
DR PRO; PR:Q8LE43; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LE43; baseline and differential.
DR Genevisible; Q8LE43; AT.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IDA:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31992; PTHR31992; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..288
FT /note="Dof zinc finger protein DOF2.1"
FT /id="PRO_0000074272"
FT ZN_FING 48..102
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 97..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 50
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 53
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 75
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 78
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 201
FT /note="M -> V (in Ref. 4; AAM62856)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 31782 MW; 76DAD69708B6A6CD CRC64;
MDPEQEISNE TLETILVSST KGSNNNNKKM EEEMKKKVSR GELGGEAQNC PRCESPNTKF
CYYNNYSLSQ PRYFCKSCRR YWTKGGTLRN VPVGGGCRRN KRSSSSAFSK NNNNKSINFH
TDPLQNPLIT GMPPSSFGYD HSIDLNLAFA TLQKHHLSSQ ATTPSFGFGG DLSIYGNSTN
DVGIFGGQNG TYNNSLCYGF MSGNGNNNQN EIKMASTLGM SLEGNERKQE NVNNNNNNSE
NPSKVFWGFP WQMTGDSAGV VPEIDPGRES WNGMVSSWNN GLLNTPLV
