DOF32_ARATH
ID DOF32_ARATH Reviewed; 245 AA.
AC Q9M1E6; Q9SUJ4;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Dof zinc finger protein DOF3.2 {ECO:0000303|PubMed:12475498};
DE Short=AtDOF3.2 {ECO:0000303|PubMed:12475498};
DE AltName: Full=Protein PHLOEM EARLY DOF6 {ECO:0000303|PubMed:30626969};
GN Name=DOF3.2 {ECO:0000303|PubMed:12475498};
GN Synonyms=DOF6 {ECO:0000303|PubMed:22155632};
GN OrderedLocusNames=At3g45610 {ECO:0000312|Araport:AT3G45610};
GN ORFNames=F9K21.190 {ECO:0000312|EMBL:CAB75490.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24;
RA Malkowski B., Plesch G., Mueller-Roeber B.;
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [6]
RP FUNCTION, INTERACTION WITH TCP14, AND DEVELOPMENTAL STAGE.
RX PubMed=22155632; DOI=10.1093/jxb/err388;
RA Rueda-Romero P., Barrero-Sicilia C., Gomez-Cadenas A., Carbonero P.,
RA Onate-Sanchez L.;
RT "Arabidopsis thaliana DOF6 negatively affects germination in non-after-
RT ripened seeds and interacts with TCP14.";
RL J. Exp. Bot. 63:1937-1949(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND INDUCTION BY
RP CYTOKININ.
RX PubMed=30626969; DOI=10.1038/s41586-018-0839-y;
RA Miyashima S., Roszak P., Sevilem I., Toyokura K., Blob B., Heo J.-O.,
RA Mellor N., Help-Rinta-Rahko H., Otero S., Smet W., Boekschoten M.,
RA Hooiveld G., Hashimoto K., Smetana O., Siligato R., Wallner E.-S.,
RA Maehoenen A.P., Kondo Y., Melnyk C.W., Greb T., Nakajima K., Sozzani R.,
RA Bishopp A., De Rybel B., Helariutta Y.;
RT "Mobile PEAR transcription factors integrate positional cues to prime
RT cambial growth.";
RL Nature 565:490-494(2019).
CC -!- FUNCTION: Transcription factor that negatively affects seed germination
CC and opposes TCP14 function in the regulation of a specific set of
CC abscisic acid-related genes (PubMed:22155632). The PEAR proteins (e.g.
CC DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) activate gene
CC expression that promotes radial growth of protophloem sieve elements
CC (PubMed:30626969). {ECO:0000269|PubMed:22155632,
CC ECO:0000269|PubMed:30626969}.
CC -!- SUBUNIT: Interacts with TCP14. {ECO:0000269|PubMed:22155632}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00071}.
CC -!- TISSUE SPECIFICITY: The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2,
CC DOF1.1, DOF5.6 and DOF5.3) form a short-range concentration gradient
CC that peaks at protophloem sieve elements (PSE).
CC {ECO:0000269|PubMed:30626969}.
CC -!- DEVELOPMENTAL STAGE: The transcript levels of the gene accumulate in
CC dry seeds and decay gradually during after-ripening and also upon seed
CC imbibition. {ECO:0000269|PubMed:22155632}.
CC -!- INDUCTION: By cytokinin in procambium. {ECO:0000269|PubMed:30626969}.
CC -!- DISRUPTION PHENOTYPE: The pear1 pear2 dof6 tmo6 quadruple mutant plants
CC showed a uniform reduction in radial growth, associated with
CC compromised symplastic trafficking. {ECO:0000269|PubMed:30626969}.
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DR EMBL; AJ243033; CAB51901.1; -; mRNA.
DR EMBL; AL138657; CAB75490.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78050.1; -; Genomic_DNA.
DR EMBL; AY062783; AAL32861.1; -; mRNA.
DR EMBL; BT006284; AAP13392.1; -; mRNA.
DR PIR; T47501; T47501.
DR RefSeq; NP_190147.1; NM_114430.3.
DR AlphaFoldDB; Q9M1E6; -.
DR BioGRID; 9023; 8.
DR IntAct; Q9M1E6; 3.
DR STRING; 3702.AT3G45610.1; -.
DR PaxDb; Q9M1E6; -.
DR PRIDE; Q9M1E6; -.
DR EnsemblPlants; AT3G45610.1; AT3G45610.1; AT3G45610.
DR GeneID; 823703; -.
DR Gramene; AT3G45610.1; AT3G45610.1; AT3G45610.
DR KEGG; ath:AT3G45610; -.
DR Araport; AT3G45610; -.
DR TAIR; locus:2085697; AT3G45610.
DR eggNOG; ENOG502RB9Y; Eukaryota.
DR HOGENOM; CLU_036438_2_1_1; -.
DR InParanoid; Q9M1E6; -.
DR OMA; NISNEQM; -.
DR OrthoDB; 1111544at2759; -.
DR PhylomeDB; Q9M1E6; -.
DR PRO; PR:Q9M1E6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1E6; baseline and differential.
DR Genevisible; Q9M1E6; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0090057; P:root radial pattern formation; IGI:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31992; PTHR31992; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..245
FT /note="Dof zinc finger protein DOF3.2"
FT /id="PRO_0000074278"
FT ZN_FING 40..94
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 15..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..118
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 42
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 45
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 67
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 18
FT /note="T -> S (in Ref. 1; CAB51901)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 26958 MW; E7AA65580BF03C3A CRC64;
MDYSSMHQNV MGVSSCSTQD YQNQKKPLSA TRPAPPEQSL RCPRCDSTNT KFCYYNNYSL
SQPRYFCKSC RRYWTKGGIL RNIPIGGAYR KHKRSSSATK SLRTTPEPTM THDGKSFPTA
SFGYNNNNIS NEQMELGLAY ALLNKQPLGV SSHLGFGSSQ SPMAMDGVYG TTSHQMENTG
YAFGNGGGGM EQMATSDPNR VLWGFPWQMN MGGGSGHGHG HVDQIDSGRE IWSSTVNYIN
TGALL
