DOF46_ARATH
ID DOF46_ARATH Reviewed; 342 AA.
AC Q8LAP8; O22984; Q94C22; Q9SU49;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Dof zinc finger protein DOF4.6 {ECO:0000303|PubMed:12475498};
DE Short=AtDOF4.6 {ECO:0000303|PubMed:12475498};
GN Name=DOF4.6 {ECO:0000303|PubMed:12475498};
GN OrderedLocusNames=At4g24060 {ECO:0000312|Araport:AT4G24060};
GN ORFNames=T19F6.13 {ECO:0000312|EMBL:CAB51649.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [6]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20563990; DOI=10.1387/ijdb.093006jg;
RA Gardiner J., Sherr I., Scarpella E.;
RT "Expression of DOF genes identifies early stages of vascular development in
RT Arabidopsis leaves.";
RL Int. J. Dev. Biol. 54:1389-1396(2010).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00071}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LAP8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LAP8-2; Sequence=VSP_011869;
CC -!- TISSUE SPECIFICITY: Accumulates in the stele.
CC {ECO:0000269|PubMed:20563990}.
CC -!- DEVELOPMENTAL STAGE: Expressed at preprocambial stages first in wide
CC domains, and later confined to sites of vein development.
CC {ECO:0000269|PubMed:20563990}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB51649.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81324.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002343; AAB63618.1; -; Genomic_DNA.
DR EMBL; AL109619; CAB51649.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161560; CAB81324.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84846.1; -; Genomic_DNA.
DR EMBL; AY037220; AAK59820.1; -; mRNA.
DR EMBL; BT008771; AAP68210.1; -; mRNA.
DR EMBL; AY087686; AAM65223.1; -; mRNA.
DR PIR; T13450; T13450.
DR RefSeq; NP_567693.1; NM_118538.4. [Q8LAP8-1]
DR AlphaFoldDB; Q8LAP8; -.
DR BioGRID; 13795; 1.
DR IntAct; Q8LAP8; 1.
DR STRING; 3702.AT4G24060.1; -.
DR PaxDb; Q8LAP8; -.
DR PRIDE; Q8LAP8; -.
DR ProteomicsDB; 222122; -. [Q8LAP8-1]
DR EnsemblPlants; AT4G24060.1; AT4G24060.1; AT4G24060. [Q8LAP8-1]
DR GeneID; 828506; -.
DR Gramene; AT4G24060.1; AT4G24060.1; AT4G24060. [Q8LAP8-1]
DR KEGG; ath:AT4G24060; -.
DR Araport; AT4G24060; -.
DR TAIR; locus:2134981; AT4G24060.
DR eggNOG; ENOG502QPN9; Eukaryota.
DR HOGENOM; CLU_036438_5_1_1; -.
DR InParanoid; Q8LAP8; -.
DR OMA; KPCNTNT; -.
DR PhylomeDB; Q8LAP8; -.
DR PRO; PR:Q8LAP8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LAP8; baseline and differential.
DR Genevisible; Q8LAP8; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31992; PTHR31992; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..342
FT /note="Dof zinc finger protein DOF4.6"
FT /id="PRO_0000074289"
FT ZN_FING 53..107
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 21..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 55
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 58
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 80
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 83
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT VAR_SEQ 1..138
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_011869"
FT CONFLICT 33
FT /note="P -> T (in Ref. 4; AAM65223)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="S -> F (in Ref. 4; AAM65223)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="A -> T (in Ref. 4; AAM65223)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="Q -> E (in Ref. 4; AAM65223)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 342 AA; 37348 MW; 8FAD64D9F18E7897 CRC64;
MDTAQWPQEI VVKPLEEIVT NTCPKPQPQP LQPQQPPSVG GERKARPEKD QAVNCPRCNS
TNTKFCYYNN YSLTQPRYFC KGCRRYWTEG GSLRNIPVGG GSRKNKRSHS SSSDISNNHS
DSTQPATKKH LSDHHHHLMS MSQQGLTGQN PKFLETTQQD LNLGFSPHGM IRTNFTDLIH
NIGNNTNKSN NNNNPLIVSS CSAMATSSLD LIRNNSNNGN SSNSSFMGFP VHNQDPASGG
FSMQDHYKPC NTNTTLLGFS LDHHHNNGFH GGFQGGEEGG EGGDDVNGRH LFPFEDLKLP
VSSSSATINV DINEHQKRGS GSDAAATSGG YWTGMLSGGS WC
