DOF53_ARATH
ID DOF53_ARATH Reviewed; 257 AA.
AC Q84TE9; Q0WRV9; Q8LDE1; Q9LSS6;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Dof zinc finger protein DOF5.3 {ECO:0000303|PubMed:12475498};
DE Short=AtDOF5.3 {ECO:0000303|PubMed:12475498};
DE AltName: Full=Protein PHLOEM EARLY DOF TMO6 {ECO:0000303|PubMed:30626969};
DE AltName: Full=Protein TARGET OF MONOPTEROS 6 {ECO:0000303|PubMed:20220754};
DE Short=Protein TARGET OF MP 6 {ECO:0000303|PubMed:20220754};
GN Name=DOF5.3 {ECO:0000303|PubMed:12475498};
GN Synonyms=TMO6 {ECO:0000303|PubMed:20220754};
GN OrderedLocusNames=At5g60200 {ECO:0000312|Araport:AT5G60200};
GN ORFNames=F15L12.9 {ECO:0000312|EMBL:BAA97501.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [8]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20563990; DOI=10.1387/ijdb.093006jg;
RA Gardiner J., Sherr I., Scarpella E.;
RT "Expression of DOF genes identifies early stages of vascular development in
RT Arabidopsis leaves.";
RL Int. J. Dev. Biol. 54:1389-1396(2010).
RN [9]
RP INDUCTION BY MONOPTEROS, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=20220754; DOI=10.1038/nature08836;
RA Schlereth A., Moller B., Liu W., Kientz M., Flipse J., Rademacher E.H.,
RA Schmid M., Jurgens G., Weijers D.;
RT "MONOPTEROS controls embryonic root initiation by regulating a mobile
RT transcription factor.";
RL Nature 464:913-916(2010).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND INDUCTION BY
RP CYTOKININ.
RX PubMed=30626969; DOI=10.1038/s41586-018-0839-y;
RA Miyashima S., Roszak P., Sevilem I., Toyokura K., Blob B., Heo J.-O.,
RA Mellor N., Help-Rinta-Rahko H., Otero S., Smet W., Boekschoten M.,
RA Hooiveld G., Hashimoto K., Smetana O., Siligato R., Wallner E.-S.,
RA Maehoenen A.P., Kondo Y., Melnyk C.W., Greb T., Nakajima K., Sozzani R.,
RA Bishopp A., De Rybel B., Helariutta Y.;
RT "Mobile PEAR transcription factors integrate positional cues to prime
RT cambial growth.";
RL Nature 565:490-494(2019).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence (By similarity). The PEAR proteins (e.g.
CC DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) activate gene
CC expression that promotes radial growth of protophloem sieve elements
CC (PubMed:30626969). {ECO:0000250|UniProtKB:Q9M2U1,
CC ECO:0000269|PubMed:30626969}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00071}.
CC -!- TISSUE SPECIFICITY: The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2,
CC DOF1.1, DOF5.6 and DOF5.3) form a short-range concentration gradient
CC that peaks at protophloem sieve elements (PSE) (PubMed:30626969).
CC Accumulates in the stele (PubMed:20563990).
CC {ECO:0000269|PubMed:20563990, ECO:0000269|PubMed:30626969}.
CC -!- DEVELOPMENTAL STAGE: In embryos, present in cells relevant for root
CC initiation and later in vascular tissues. At the globular stage,
CC accumulates in cells adjacent to the hypophysis (extra-embryonic cell
CC specified to become the founder cell of the primary root meristem)
CC (PubMed:20220754). Expressed at preprocambial stages first in wide
CC domains, and later confined to sites of vein development. In young
CC seedlings, first observed in the central region of leaves primordia,
CC and later strongly expressed at sites of midvein, first and second
CC loops, and higher-order veins (PubMed:20563990).
CC {ECO:0000269|PubMed:20220754, ECO:0000269|PubMed:20563990}.
CC -!- INDUCTION: By cytokinin in procambium (PubMed:30626969). Induced by the
CC transcription factor MONOPTEROS (MP) in cells relevant for root
CC initiation, and later in vascular tissues and hypophysis
CC (PubMed:20220754). {ECO:0000269|PubMed:20220754,
CC ECO:0000269|PubMed:30626969}.
CC -!- DISRUPTION PHENOTYPE: The pear1 pear2 tmo6 triple mutant variably
CC displays reduced radial growth. The pear1 pear2 dof6 tmo6 quadruple
CC mutant plants showed a greater uniform reduction in radial growth,
CC associated with compromised symplastic trafficking.
CC {ECO:0000269|PubMed:30626969}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM63264.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA97501.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB026632; BAA97501.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97292.1; -; Genomic_DNA.
DR EMBL; AK228185; BAF00140.1; -; mRNA.
DR EMBL; BT005866; AAO64801.1; -; mRNA.
DR EMBL; AY086054; AAM63264.1; ALT_INIT; mRNA.
DR EMBL; AB493803; BAH30641.1; -; mRNA.
DR RefSeq; NP_568920.1; NM_125413.4.
DR AlphaFoldDB; Q84TE9; -.
DR BioGRID; 21386; 4.
DR STRING; 3702.AT5G60200.1; -.
DR PaxDb; Q84TE9; -.
DR PRIDE; Q84TE9; -.
DR ProteomicsDB; 222125; -.
DR EnsemblPlants; AT5G60200.1; AT5G60200.1; AT5G60200.
DR GeneID; 836142; -.
DR Gramene; AT5G60200.1; AT5G60200.1; AT5G60200.
DR KEGG; ath:AT5G60200; -.
DR Araport; AT5G60200; -.
DR TAIR; locus:2144030; AT5G60200.
DR eggNOG; ENOG502RB9Y; Eukaryota.
DR HOGENOM; CLU_036438_2_1_1; -.
DR OMA; MNGESFG; -.
DR OrthoDB; 1111544at2759; -.
DR PhylomeDB; Q84TE9; -.
DR PRO; PR:Q84TE9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q84TE9; baseline and differential.
DR Genevisible; Q84TE9; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IDA:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0048364; P:root development; IEP:TAIR.
DR GO; GO:0090057; P:root radial pattern formation; IGI:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31992; PTHR31992; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..257
FT /note="Dof zinc finger protein DOF5.3"
FT /id="PRO_0000074293"
FT ZN_FING 55..109
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 23..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 57
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 60
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 82
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 85
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 31
FT /note="P -> Q (in Ref. 5; AAM63264)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="V -> AAA (in Ref. 5; AAM63264)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 257 AA; 28201 MW; E9A29940F86EB693 CRC64;
MDHLLQHQDV FGNYNKAREA MGLSYSSNPT PLDNDQKKPS PATAVTRPQP PELALRCPRC
DSTNTKFCYY NNYSLTQPRY FCKSCRRYWT KGGTLRNIPV GGGCRKNKRS TSSAARSLRT
TPEPASHDGK VFSAAGFNGY SNNEHIDLSL AFALLNKQHP GSSSQLGFHS ELGSSHQSDM
EGMFGTSQQK ENATYAFGNG SSGLGDPSRV LWGFPWQMNG ESFGMMNIGG GGGHVDQIDS
GREMWTNMNY INSGALM
